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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Expression and purification of kinase domain of ASK1 kinase.
 Bártová, Hana ; Obšil, Tomáš (advisor) ; Gryčová, Lenka (referee)
The goal of this diploma thesis was to find optimal conditions for expression of ASK1 kinase in prokaryotic expression system and to optimize purification protocol which enables preparing of milligram amounts of stable and soluble protein. Different conditions of expression were tested in E. coli cells including temperature of expression, cultivation medium or the length of induction. Different methods of purification were tested during the development of the purification protocol. The final protocol is based on chelate chromatography followed by gel permeation chromatography. The result of the diploma thesis is a protocol that allows preparing 1 mg of pure ASK1 kinase from 1 liter of medium.
Detailed record
Expression and purification of kinase domain of ASK1 kinase.
 Bártová, Hana ; Gryčová, Lenka (referee) ; Obšil, Tomáš (advisor)
The goal of this diploma thesis was to find optimal conditions for expression of ASK1 kinase in prokaryotic expression system and to optimize purification protocol which enables preparing of milligram amounts of stable and soluble protein. Different conditions of expression were tested in E. coli cells including temperature of expression, cultivation medium or the length of induction. Different methods of purification were tested during the development of the purification protocol. The final protocol is based on chelate chromatography followed by gel permeation chromatography. The result of the diploma thesis is a protocol that allows preparing 1 mg of pure ASK1 kinase from 1 liter of medium.
Detailed record

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