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Evaluation of tooth preparation using Er: YAG laser in comparison with other preparation methods
Bučková, Michaela ; Dostálová, Taťjana (advisor) ; Racek, Jaroslav (referee) ; Mazánek, Jiří (referee) ; Smrčka, Pavel (referee)
Er: YAG laser is a new method of preparation that promises gentler and more accurate dental treatment. Its specific characteristics is large absorption of the beam in the water and a small absorption in the tissue without the thermal effect. Therefore the treatment is safer and effectiver. The research has the aim in its theoretical - cognitive part to assess its advantages in comparison with treaditional treatment method - drill. As an essential element will be evaluation of caries lesion in vitro and in vivo. The size of the cavity will be judged on, the shape of developing a smear layer and retention surface in relation to the filling and the . The extracted teeth will be scanned in electronic microscope to assess the formation of contact and non-contact preparation, focusing on the shape and size of the prepared lesion in relation to modern filling materials - low viscosity composites. Extensive research supported by the Ministry of Health (IGA : 9991-4 and 13351-4) that focuses on the the use of various therapeutic methods in the treatment of special needs patients. Dental treatment of a special needs patients is expensive and time-consuming than conventional dental treatment. Therefore, clinical application of theoretical cognitive part will create a electronical support system for the...
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Bird Taxidermy in Selected Bird Species
Hepnarová, Věra ; Řezníček, Jan (advisor) ; Mazalová, Jana (referee)
The thesis briefly describes some techniques of preparation which should lead to creating ornithological dermoplastic preparations and their use in education and biology. These techniques are shown on several bird species. The thesis can be also used as a instruction for creating ornithological dermoplastic bird preparations. It shows some problems of preparation technice. The main goal of the thesis is creating a real ornithological preparations which are, used for education on Pedagogical Faculty of Charles University in Prague. Key words: teaching practices, preparation, taxidermy, birds, ornithological preparation
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Chemo-enzymatic synthesis of antiviral prodrugs
Tupec, Michal ; Stiborová, Marie (advisor) ; Indra, Radek (referee)
Lipases have been widely applied in the manufacture of food products and in some areas of the industry, nowadays they are used in synthetic organic chemistry catalyzing the hydrolytic/esterification reactions under very mild conditions in the field of protecting groups or enantiomer resolution. In this study, the commercial lipase from bacterium Pseudomonas fluorescens was immobilized using the sol-gel process into organosilicate materials with propyl, octyl or phenyl substituents. The highest hydrolytic activity was found in the enzyme on the octyl-derived carrier. The immobilized enzymes differ in their hydrolytic activities on 4-nitrophenyl esters of various lengths. Subsequent experiments revealed quite good pH stability of the enzymes in a buffer (incubations in pH 3 through pH 11), as well as good temperature stability in isooctane (incubations at up to 100 řC). The majority of organic solvents seem to have no substantial effect on the lipase activity. The biocatalytic properties were studied on a model compound from the group of the acyclic nucleoside analogues - 9-(2',3'-dihydroxypropyl)adenine (DHPA). It was found for example that the best acyl donors are vinyl esters, that the lipase shows a preference towards longer vinyl esters, that the reaction proceeds faster in non-polar solvents or that it...
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Vertebrate Skeletal Mounts
Poláchová, Tereza ; Řezníček, Jan (advisor) ; Mazalová, Jana (referee)
The topic of this thesis is dissection of vertebrate skeletons while using available and appropriate methods. The theoretical part is prepared based on Czech and foreign sources. For this part, there were used print publications, articles from journals and some Internet sources. It summarizes theoretical knowledge of the occurrence and anatomical structure of vertebrates. The practical part includes methodology involving collection, preservation and actual processing of animals. In detail, there are described possible methods of work that are evaluated and compared in the closing part. These proposed procedures are used for the selected representatives of vertebrates, in the individual chapter called - Results. The outcome of this thesis is a detailed guide with the procedures of chosen methods and preserved skeletons with a description of their characteristics. Powered by TCPDF (www.tcpdf.org)
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Vertebrate Taxidermy
Mazalová, Jana ; Řezníček, Jan (advisor) ; Odcházelová, Tereza (referee)
TITLE: Manufacturing taxidermy mounts and their use as teaching aids AUTHOR: Jana Mazalová DEPARTMENT: Department of Biology and Environmental Studies SUPERVISOR: RNDr. Jan Řezníček PhD. ABSTRAKT: The main subject of this thesis is focused on preparation of taxidermal mounts with use of mammal bones and skins and their use as teaching aids. The target group are school children both of elementary and secondary schools. The thesis describes methods of bone preservation and cleaning, skin tanning and preparation of taxidermal mounts. I deal in it also with preparation of large vertebrates' skulls and preparation of taxidermal mounts. The procedures are described in details and the following chapters include photos of dissections made by me. Preparation of 4 bird and 6 mammal species is described. For each description, search of recent literature and PowerPoint presentation is included. KEYWORDS: preparation, taxidermal mounts, skin, vertebrates' skulls
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Úpravy rostlin a jejich využití v historickém interiéru
Valenta, Jiří
The subject of this final thesis was to study the techniques used to extend the life of plants, in practice to test and evaluate them. The obtained material is used to design an arrangement for specific historical interiors of Znojmo castle. The third task was to measure the temperature and humidity in one of the rooms where the arrangememnt was placed. The thesis includes a supplement with photographs of plants preserved with various techniques and examples of period interiors with installed floral arrangaments.
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The influence of the fixation of water bugs (Heteroptera: Nepomorpha) on the results of their growth and variability
SVOBODOVÁ, Kristýna
The main task of this thesis was to investigate the influence of the preservation by ethyl alcohol and dry mounting on the morphometric characteristics of water bugs´ and to test the hypothesis that live individuals and individuals preserved by ethyl alcohol and dry mounted (both groups deal with individuals of the same species, sex and developmental stage) have the same morphometric parameters. The next task was to determine the effect of preparation by ethyl alcohol and dry mounting on the change of weight caused by different way of individuals´ preparation. Two model species of water bugs were chosen for this study: creeping water bug Ilyocoris cimicoides (Naucoridae) and backswimmer Notonecta glauca (Notonectidae). Weight and twelve other morphometric body characters were measured in adults of both sexes of both species. All data and their differences were tested by Student's paired t-test or nonparametric Wilcoxon test. The level of significance p = 0,05 was used for the decision whether the difference before and after preservation by ethyl alcohol and dry mounting is or is not statistically significant. Both preservation by ethyl alcohol and dry mounting influence only the body weight of treated individuals. Other morphometric characters are more or less independent on the way of preservation of water bugs´ individuals in collections. In this work, we also tested the effect of the Dyar rule for the increase in weight and size between different developmental stages of both species studied. Dyar rule for the evolution of both species was not verified. This project was supported by the grant of the Grant Agency of the Czech Republic No. P505/10/0096.
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