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Characterisation of proteins whose degradation is necessary for normal course of embryonic genome activation in mammals
Svobodová, Nika ; Toralová, Tereza (advisor) ; Krejčová, Tereza (referee)
Early embryonic development is controlled by maternal mRNAs and proteins synthesized during oogenesis. A key period for preimplantation development is the transition from maternal control of development to embryonic control. This process, known as maternal-to-zygotic transition (MZT), requires the coordinated degradation of accumulated maternal mRNAs and proteins and subsequent embryonic genome activation (EGA). Maternal mRNAs are gradually removed from the embryo, but the degradation of maternal proteins is not well understood yet. In mammals, only a few proteins are known whose degradation is necessary for normal course of EGA. The activation of the embryonic genome is closely related to the reorganization of chromatin structure. The initiation of gene expression requires the loosening of chromatin at the gene region and the presence of appropriate transcription factors. Maternal proteins that need to be degraded for the normal course of EGA are involved in regulating chromatin structure and the translocation of necessary factors. This thesis aims to characterize selected proteins (PIASy, CBX5, TAB1 and H1FOO) and describe the role they play during the transition from maternal to embryonic control of development. While PIASy, CBX5, and H1FOO need to be degraded to loosen the chromatin structure...
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Rypoš lysý a myš domácí jako modelové organismy reprodukčního stárnutí
KOPECKÁ, Eva
The aim of this study is to demonstrate the naked mole rat as a new possible model of reproductive aging. It is also pointing out the reproductive problems and diseases associated with increasing age in women. Furthermore, the study wants to introduce the role of mTOR in mouse embryonic development in relation to maternal age and decrease in fertility in a view of number of oocytes in mouse, guinea pig and naked mole rat.
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Role genu Socs3 v preimplantačním vývoji myších embryí
TOMÁNKOVÁ, Jana
The Socs3 gene is important for the postimplantation development of mouse embryos, but not much is known about its effect on preimplantation development. The aim of this study was to determine the effect of Socs3 gene in preimplantation development of mouse embryos, specifically on proliferation at E3.5 timepoint, at the time of seggregation of cell lineages trofoectoderm and inner cell mass.
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