|
|
Development of a molecular methodology for the identification of insects used for human consumption
MÜLLEROVÁ, Veronika
Insect is often recognized as foodstuff of the future. An important requirement for insect use as a foodstuff is the development of an accurate and reliable method of insect detection. The goal of the bachelor thesis was to develop a molecular method that could be used for detecting the mealworm (Tenebrio molitor), an EU-approved species, in foodstuffs. The experimental part of the thesis included optimalization of mealworm DNA extraction. Specificity test of the method was performer on the superworm (Zophobas morio), which is not approved for consumption in the EU. DNA was extracted using 3 different methods. The optimal method achieving highest DNA yield and purity was DNA extraction with CTAB-PVP. Following DNA extraction, optimalization for PCR mix and cycle was peformed, which included optimalization of primer annealing. Results were visualised by gel electrophoresis. The optimized method is accurate, specific and appropriate for routine detection of the mealworm in foodstuffs.
|
|
|
Innovation of device for mealworms breeding
Dvorský, Adam ; Búran, Martin (referee) ; Adámek, Martin (advisor)
The task of the work was to get familiar to the reader with the possibilities and conditions of edible insect breeding in the Czech Republic, focusing on a selected species of darkling beetle (Tenebrio molitor). Design optimization of breeding conditions for different stages of breeding, with the possibility of accelerating breeding. Another part of the work was to get acquainted with the properties of the microcontroller series Espressif ESP32 and its use in the areas of simple product management. From these identified conditions, a breeding station was designed and gradually implemented in the second half of the work, which was controlled by this microcontroller company Espressif ESP32. The work describes the proposed components for measuring and regulating breeding conditions with a focus on temperature, humidity, food and fluid dosing. The penultimate chapter mentions experimental measurements of regulated conditions with evaluation of measured data. The conclusion is the evaluation of the solution and its other possibilities.
|
|
|
Device for measurement and regulation of edible insect breeding conditions
Dvorský, Adam ; Búran, Martin (referee) ; Adámek, Martin (advisor)
This work focuses on the design and realization of a measuring and regulatory system for successful breeding of edible insects with a focus on the mealworm (Tenebrio molitor). For the correct setting of the system, the first part of the work is detected and described the general conditions of insect breeding. The description is mainly focused on the mealworm, its development and basic breeding conditions (temperature, humidity, etc.). This information is further used for the development of breeding equipment, the basic concept of which is further established at work. The device is controlled by espressif's ESP32 microcontroller. The work acquaints with its programming and the connection and programming of basic components for the proposed system. Experimental measurements verify the functionality of measurements and regulation in the breeding box. The regulation is accurate to tenths to hundredths of ° C.Last but not least, the design is compared with existing devices.
|
|
|
Stanovení profilu mastných kyselin v hmyzích surovinách modifikovaných výživovými přídavky jedlých olejů a vitamínu D k základnímu krmivu hmyzu
Tomanová, Adéla
The thesis deals with the profile of fatty acids in the body of the larval stage of the Tenebrio molitor, and how the content of fatty acids in the body of this insect could be affected by the type of food which they consume. The theoretical part is devoted to legislation dealing with the issue of edible insects in the Czech Republic and the EU, a general anatomical description of the insect body, characteristics of representatives of approved edible insects including their nutritional aspects, advantages and disadvantages of insect consumption, industrial processing of insects and food neophobias. There are also included the general characteristics and distribution of fatty acids. The practical part is dedicated to the methodology of fattening own samples of larvae of Tenebrio molitor fed with basic feed enriched with vegetable oils and vitamin D, as well as the processes for determining the fatty acid profile in their bodies and evaluating the obtained results and discussion.
|
|
|
Trendy ve využití hmyzí suroviny v potravinách
Polák, Daniel
This bachelor thesis deals with the use of edible insects material in the food industry. Around 2000 edible insect species have been recorded worldwide and are commonly consumed in Africa, Asia and Oceania. In recent years, edible insects have also become a major topic of discussion in the Western world. In the European Union, legislative steps have been taken to categorise certain species of the edible insects as food. On the European market, products made from the yellow mealworm, the house cricket, the migratory locust and the lesser mealworm can be found. These edible insects are a rich source of protein, fat, vitamins and minerals. It also contains the fibre chitin which is associated with lower digestibility of insect products and may also act as an allergen. Some nutritional substances may be subject to the development of innovative technologies. For example, proteins from the migratory anemone have the potential for the development of edible packaging or chitin fibre may be another alternative source to obtain chitosan.
|
|
|
Vliv krmiv na výnos jatečního materiálu hmyzu (Tenebrio molitor)
MUCHKA, Lukáš
Bachelor thesis consists of theoretical and practical part. The theoretical part of this work deals with the consumption of edible insects both in the world and in the Czech Republic. Furthermore, the advantages and disadvantages of the consumption of edible insects and their nutritional value are described. It also includes characteristics of the mealworm (Tenebrio molitor). An important part of the thesis is the practical part, whose aim was to determine the effect of different types of protein feed in the breeding of mealworms. Here their effects on the weight yield of insect slaughter material were compared. The experiment was carried out using four types of protein feed: plant protein (soy), animal protein (dehydrated eggs), complex feed (feed for aquarium fish) and mix of the above feed. Breeding took place in the months of October - December 2019. Two experimental generations of adult insects were bred for egg production. The subsequent egg separation produced separate generations of insects which were fed for fifty-two days. The experiment found that the best results in the breeding of mealworms are achieved by insects, which are fed with a mix of feed in which the largest amount was harvested. Successful breeding of edible insects, however, requires the interplay of several conditions. The choice of feed is one of the important criteria to be addressed. This work is a basic study which can be followed by more detailed experiments.
|
|
|
Innovation of device for mealworms breeding
Dvorský, Adam ; Búran, Martin (referee) ; Adámek, Martin (advisor)
The task of the work was to get familiar to the reader with the possibilities and conditions of edible insect breeding in the Czech Republic, focusing on a selected species of darkling beetle (Tenebrio molitor). Design optimization of breeding conditions for different stages of breeding, with the possibility of accelerating breeding. Another part of the work was to get acquainted with the properties of the microcontroller series Espressif ESP32 and its use in the areas of simple product management. From these identified conditions, a breeding station was designed and gradually implemented in the second half of the work, which was controlled by this microcontroller company Espressif ESP32. The work describes the proposed components for measuring and regulating breeding conditions with a focus on temperature, humidity, food and fluid dosing. The penultimate chapter mentions experimental measurements of regulated conditions with evaluation of measured data. The conclusion is the evaluation of the solution and its other possibilities.
|
|
|
Device for measurement and regulation of edible insect breeding conditions
Dvorský, Adam ; Búran, Martin (referee) ; Adámek, Martin (advisor)
This work focuses on the design and realization of a measuring and regulatory system for successful breeding of edible insects with a focus on the mealworm (Tenebrio molitor). For the correct setting of the system, the first part of the work is detected and described the general conditions of insect breeding. The description is mainly focused on the mealworm, its development and basic breeding conditions (temperature, humidity, etc.). This information is further used for the development of breeding equipment, the basic concept of which is further established at work. The device is controlled by espressif's ESP32 microcontroller. The work acquaints with its programming and the connection and programming of basic components for the proposed system. Experimental measurements verify the functionality of measurements and regulation in the breeding box. The regulation is accurate to tenths to hundredths of ° C.Last but not least, the design is compared with existing devices.
|
guest ::
