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Physiological and pathophysiological role of GCPII in the body
Sedlák, František ; Konvalinka, Jan (advisor) ; Klener, Pavel (referee) ; Smetana, Karel (referee)
Glutamate carboxypeptidase II (GCPII) is a metalloprotease responsible for cleaving the neurotransmitter N-acetyl-aspartyl-glutamate in the central nervous system to N-acetyl aspartate and glutamate. At the same time, in the human small intestine, it facilitates folate absorption by cleaving γ-linked glutamate from folyl-poly-γ-glutamate. In humans, GCPII is also expressed in a number of other organs (e.g., kidney and prostate) and tumors, where its physiological function is unknown. In an attempt to characterize the physiological function of the enzyme, we first characterized the commercially available monoclonal antibodies against GCPII. Further, we developed a fully synthetic replacement based on a hydrophilic polymer with bound GCPII inhibitors. We evaluated the suitability of using a murine biomodel to study GCPII function in vivo. We found the difference in GCPII expression profile in mouse and human. We did not observe GCPII in either the mouse prostate or small intestine. To assess physiological and pathophysiological functions of the enzyme we analyzed a GCPII-deficient mouse model. Apart from the observation of enlarged seminal vesicles in older males, we did not detect any other obvious phenotype. Similarly, we confirmed that GCPII cannot cleave amyloid peptides (Aβ1-40 and Aβ1-42)....
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Physical examinations for adults performed by nurse in practitioner´s ordination.
MANČUŠKOVÁ, Zdenka
This bachelor thesis deals with physical examination, the monitoring of physiological functions and the recording of cardiographs by nurses in private practice examination rooms. The thesis contains two parts. The first, and theoretical, part of the thesis is focused on the explanation of certain terms and a description of what it means to provide a physical examination, monitoring of physiological functions and recording cardiographs. The physical examination is focused around human senses. Sight (inspection), hearing (auscultation), touching (palpation), knocking (percussion), per rectum examination, and smelling are all considered methods of examination. Body temperature, breathing, pulse and blood pressure are considered physiological or vital functions. The electrocardiogram signifies cardiac activity in the form of a line, which is drawn by a gadget called an electrocardiogram (ECG). Activities executed by nurses in a private practice exam rooms can be divided into four groups: preventative, diagnostic, medical and administrative group. A nurse provides patients with medical educative materials, samples biological material, monitors clients physiological functions, gives injections, changes bandages, sends preventative examination invitations, orders medical material and equipment etc. The second part contains investigative research. The empirical investigation was performed using the qualitative method in a form of a semi structured interview and direct observation during the interview. In an interview with eight nurses, who all work in private practices, the nurses were asked 15 basic questions. The nurses were observed as they each examined a separate patient. The observation was done based on 32 observation criteria. The observation results were compared with the results of the interviews. Based on their close relation to physical examination, monitoring of physiological functions and cardiograph was added.
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The monitoring of physiological functions at Anesteziology and Intensive Care and Intensive care unit - the preparation of materials for incoming nurses
KOPECKÁ, Nina
The thesis was elaborated on the topic: FF Monitoring at ARD and ICU ? preparation of materials for newly hired nurses? The first part of the thesis introduces the ARD history and concept. It describes competences of nurses, specifics of their work, physical and psychical demands for nurses working at intensive care units. The subsequent chapters analyze not only how acceptance of newly hired nurses to ARD should be conducted, but they also deal with the complete issue of FF monitoring at ARD. The second part deals with the set goals of the thesis and related research questions. There are three goals: 1: To map the available study materials related to FF monitoring for newly hired ARD nurses. 2: To find out what FF monitoring educational materials ARD nurses would like to have available for newly hired nurses. 3: To test newly elaborated educational material related to FF monitoring at ADR in practice. And there are four reseach questions to them: 1: What educational material related to FF monitoring at ADR are available for newly hired ADR nurses? 2: What educational material related to FF monitoring at ADR would newly hired ARD nurses like to get? 3: What educational material related to FF monitoring at ADR would ARD nurses like to have available for newly hired nurses? 4: Is the newly prepared educational material related to FF monitoring suitable for initial training of newly hired ARD nurses? The third part of the thesis deals with work methodology and the research sample. The research was divided into three subsequent stages from December 2010 to June 2011. In-depth interview was chosen as the data collection technique. The research sample consisted of 8 respondents working at anaesthetic-resuscitation department of the České Budějovice Hospital, namely of four respondents with several years of experience and four respondents in the adaptation process. The fourth part of the thesis starts with interviews with the questioned respondents from the first research stage. They are interpreted by means of categorization tables in the thesis. Material on FF monitoring for newly hired nurses, available in Annex 2 is elaborated in the second stage of the research. The third stage of the research is dedicated to responses of the respondents to the newly elaborated educational material on FF monitoring. They were obtained by means of semi structured interviews with the respondents. The research has shown that ARD nurses are not given any educational materials on FF monitoring of sufficient quality. We have found that each of the respondents would like to obtain good quality materials on FF monitoring at his/her start at an ARD. However the respondents said that they did not know what exactly the educational material on FF monitoring should look like. All the eight respondents agree that they like the newly elaborated material on FF monitoring for new nurses from both, the content and form point of view and they would like to use it in practice. The fifth part of the thesis is dedicated to a discussion. It analyses and describes the research and its results. The conclusion of the thesis summarizes the research and the newly obtained information. All the goals of the thesis and the research questions are answered there. The thesis also includes a list of resources, abbreviations used in the text, key words and moreover the annexes elaborated within the work on the thesis.
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