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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Characterization of Photosystem I in the Red Alga Porphyridium purpureum
 KOSMAS, Panagiotis
Photosynthesis, a fundamental biochemical process on planet Earth, has passed through several steps during the course of evolution since beginning of time. Red algae represent a primitive stage of evolution of photosynthesis in eukaryotes, hence they are important for our understanding of functioning and evolution of photosynthetic energy conversion in general. In the current work, isolation and characterization of the supramolecular complex consisting of photosystem I with three antenna complexes from the red alga Porphyridium purpureum is presented. The purification consisted of sucrose gradient centrifugation followed by anion exchange and size-exclusion chromatography. The composition of the PSI-antenna supercomplex was confirmed using electrophoresis and reverse-phase chromatography. Function of the isolated supercomplex was further investigated using time-resolved absorption and fluorescence spectroscopy.
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Mobility of photosynthetic proteins
 KRAFL, Jaroslav
Mobility of pigment-protein complexes (phycobilizomes and photosystem II playing a key role in photosynthesis) was studied by FRAP method (Fluorescence Recovery After Photobleaching). FRAP represents a fluorescence based microscopy method enabling measurement of protein mobility in living systems. The protein complexes are bleached by a laser pulse. And mobility of unbleached proteins is measured as a fluorescence recovery in the bleached area. Currently we have only limited knowledge about the mobility of photosynthetic proteins. This work was aimed at optimization of the photosynthetic protein mobility measurement by FRAP. I have performed several methodological experiments which led to the successful assessment of phycobilisome and chlorophyll-containing proteins diffusion coefficients in selected red algae (Porfyridium cruentum, Cyanidium caldarium) and cyanobacteria (Synechocystis PCC6803, Acaryochloris marina). The methodology developed and validated in my thesis was then applied in further research projects.
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