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The effect of smoking and alcohol consumption on tooth decay and periodontitis in adults and the relationship between alcohol intake and visceral fat in the older adults
Vlasáková, Jana ; Šteffl, Michal (advisor) ; Jandová, Tereza (referee)
Title: The effect of cigarette smoking and alcohol consumption on dental caries and periodontitis in adults and the relationship between alcohol intake and visceral fat in the elderly. Objectives: The aim of my master thesis was to assess the influence of alcoholic beverages and cigarette smoking on the risk of dental caries and periodontitis in adults. Another aim was to assess the relationship between alcohol intake and visceral fat in the elderly. Methods: The thesis is carried out as two independent cross-sectional studies. In the first study, gender differences were tested using the non-parametric Kolmogorov- Smirnov test. Pearson's correlation coefficient was used to assess the relationship between the amount of alcohol consumed in kg and cigarettes smoked in pcs and the risk of dental caries or periodontitis. In the second study, body composition analysis was performed using the electrical bioimpedance method. Two-sample Kolmogorov-Smirnov test was used to test the hypotheses. In addition, a dot plot depicting the relationship between the amount of alcohol and visceral fat was created. Results: A total of 18 respondents took part in the first study, a survey. The results showed a high statistically significant correlation between the consumption of alcoholic beverages and the risk of dental...
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Periopathogens and systemic markers in etiology and diagnostics of periodontal and cardiovascular diseases.
Myšák, Jaroslav ; Foltán, René (advisor) ; Izakovičová Hollá, Lydie (referee) ; Markovská, Neda (referee)
Periodontitis is a disease primarily affecting tooth attachment, i.e. concerning oral cavity, however, its connection to a number of systemic diseases is apparent nowadays. Oral microorganisms and their interaction with the immune system of their host play a significant part in the etiology of this multifactorial disease. Rapid development of DNA-based diagnostic methods in the last 15 years dramatically increased the spectrum of identified oral microorganisms and promoted understanding of how particular taxons correlate with periodontal health or disease. Moreover, next generation sequencing methods also bring new possibilities to study the relationship between periodontitis and other diseases such as diabetes mellitus, cardiovascular diseases (CVD), stroke, lung infection or kidney diseases, etc. The presented work focuses on the use of sequencing methods to compare the taxonomic composition of microbiomes within oral cavity and the tissues of aortic valves in patients suffering from CVD. The characterisation of taxonomic composition of microbiome in the analysed tissues was performed using the method of 454 pyrosequencing of variable region IV-V of the bacterial 16S rDNA. The present taxons were determined by comparing the obtained sequences with the Human Oral Microbiome Database. The common...
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Complex characterization of subgingival plaque - use of modern sequencing methods in diagnostics and monitoring of treatment of periodontal diseases
Těšínská, Barbora ; Najmanová, Lucie (advisor) ; Konopásek, Ivo (referee)
Periodontitis is a multifactorial inflammatory disease which can result in a complete loss of teeth. Its main cause is the accumulation of bacteria from the dental plaque followed by massive reaction of the host immune system. It was proved that the composition of oral microbiome (OM) differs in periodontally healthy individuals and patients with periodontitis. This work aims to solve specific parts of the long-term project concerning the taxonomic composition of the OM of periodontally healthy individuals and patients with chronic and aggressive form of the disease. The OM was characterized based on 16s rDNA sequencing. It is evident from the results that the shift in the OM composition occurs prior the development of clincal signs of the disease and that the precise dental care can significantly postpone or even avoid the onset of the disease. When comparing the OM composition in individuals with chronic and aggressive periodontitis, no remarkable differences were detected to explain the faster progress of the aggressive form of the disease. This work also aimed to compare the results obtained by 454 pyrosequencing and Illumina. Both sequencing methods were found to provide statistically comparable results. Illumina MiSeq thus can be employed to build on the former results of the long-term study...
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