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Development of Grey Partridge (Perdix perdix) microsatellite markers
Vaněčková, Dominika ; Munclinger, Pavel (advisor) ; Burri, Marta (referee)
The aim of this work was the construction of set of microsatellites for the Grey Partridge (Perdix perdix). I succeeded in isolation of seven new specific microsatellites. Another microsatellite markers were passed from close relative galliform species (Galliformes). On the whole I gained 18 microsatellite loci which were used for study of population charakteristics of three partridge populations from Stodůlky, Písecko and Milešín. The analysis showed conspicuous structuredness of these populations and surprisingly low values (less then 100) of their effective population sizes. Seven of the microsatellites were chosen for analysis of paternity. Indeed I found the evidence of extrapair paternity in this putatively monogamous bird. Finally I conclude, on the basis of sequenation of mitochondrial control region that our populations belong to the western linage of mitochondrial DNA.
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Development of Grey Partridge (Perdix perdix) microsatellite markers
Vaněčková, Dominika ; Munclinger, Pavel (advisor) ; Burri, Marta (referee)
The aim of this work was the construction of set of microsatellites for the Grey Partridge (Perdix perdix). I succeeded in isolation of seven new specific microsatellites. Another microsatellite markers were passed from close relative galliform species (Galliformes). On the whole I gained 18 microsatellite loci which were used for study of population charakteristics of three partridge populations from Stodůlky, Písecko and Milešín. The analysis showed conspicuous structuredness of these populations and surprisingly low values (less then 100) of their effective population sizes. Seven of the microsatellites were chosen for analysis of paternity. Indeed I found the evidence of extrapair paternity in this putatively monogamous bird. Finally I conclude, on the basis of sequenation of mitochondrial control region that our populations belong to the western linage of mitochondrial DNA.
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Options and current practice of determining ferrets paternity (Mustela putorius furo)
Henclová, Tereza ; Sedlák, Petr (advisor) ; Vostrý, Luboš (referee)
Paternity tests dealing with the origin of individuals within breeds. It is an important parameter for those interested in the targeted livestock and lead to the improvement of their breed. This thesis deals with paternity tests used by the animals at the current level of genetic research and their potential application in ferrets form of literary analysis. The literature review is a basic characteristic of ferrets. Furthermore, there are contained basic genetic data, including molecular taxonomy, information, testing and paternity testing paternity insight into carnivores (Carnivora). In the discussion are discussed possibilities of using this test in ferrets.
In addressing the issue of the literature, I found that at present is carried out by PCR (polymerase chain reaction), which enables rapid DNA amplification. Using this method is performed comparing STR trio mother-baby-father. Preferably STR polymorphism is of good resolution, high variability and easy amplification by PCR, but the main disadvantage is the high mutation rate and limited number of markers in a single reaction (maximum 17). STR profiling results are expressed paternity index (Pi). The index is the result of paternity probability calculation that helps us express how big probability of paternity this alleged male. Equivalent expression Pi is the percentage ranging from 0% to 100%. The result is either confirming or disproving fatherhood. This problem is in ferrets yet elaborated and testing is currently not used. Within the current knowledge of the paternity test in ferrets contributed to the improvement of their breed that would reduce the risk of inbreeding, the transmission of hereditary diseases or defects in the next generation and to prevent fraudulent behavior in order to deceive the future owner.
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