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Periopathogens and systemic markers in etiology and diagnostics of periodontal and cardiovascular diseases.
Myšák, Jaroslav ; Foltán, René (advisor) ; Izakovičová Hollá, Lydie (referee) ; Markovská, Neda (referee)
Periodontitis is a disease primarily affecting tooth attachment, i.e. concerning oral cavity, however, its connection to a number of systemic diseases is apparent nowadays. Oral microorganisms and their interaction with the immune system of their host play a significant part in the etiology of this multifactorial disease. Rapid development of DNA-based diagnostic methods in the last 15 years dramatically increased the spectrum of identified oral microorganisms and promoted understanding of how particular taxons correlate with periodontal health or disease. Moreover, next generation sequencing methods also bring new possibilities to study the relationship between periodontitis and other diseases such as diabetes mellitus, cardiovascular diseases (CVD), stroke, lung infection or kidney diseases, etc. The presented work focuses on the use of sequencing methods to compare the taxonomic composition of microbiomes within oral cavity and the tissues of aortic valves in patients suffering from CVD. The characterisation of taxonomic composition of microbiome in the analysed tissues was performed using the method of 454 pyrosequencing of variable region IV-V of the bacterial 16S rDNA. The present taxons were determined by comparing the obtained sequences with the Human Oral Microbiome Database. The common...
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Knowledge of selected respondents about oral health
Barcalová, Barbora ; Hanušová, Jaroslava (advisor) ; Váchová, Alena (referee)
Diploma work with the theme Knowledge of selected respondents about oral health consists of a theoretical and a practical part and it deals with level of awareness about oral health by dental hygiene pacients. The main aim of this work is to provide a summary of information about oral health, free from myths and marketing manipulative techniques through a leaflet which is the product of this work. While creating the educational leaflet the author reflects results of a questionnaire survey which is a research tool of the practical part of the diploma thesis. Based on the answers given in the individual questionnaires, the information brochure is primarily directed to refute the most common misconceptions or beliefs of respondents, and at the same time, it was useful for obtaining information about correct oral care in both dental surgeries and e.g. at schools during lessos of health education. The theoretical part of the diploma work is devoted to basic anatomical and histological description of oral cavity, oral health in general, distinction of the terms gingiva, dental plaque or dental calculus. The hinge part is the chapter devoted to dental hygiene, in which the author deals with both profession of a dental hygienist and means of oral cavity care in the setting of the dental hygiene surgery and...
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Dental Hygiene impact on oral health guality of patients with dental implants
Pavlů, Jana ; Novotná, Jindra (advisor) ; Kulhavá, Miluše (referee)
Dental hygiene and oral health is a topic that is currently discussed.. Dental health reflects the overall health of humans and vice versa damaged teeth can significantly reduce health and quality of life. The results clearly confirm that the largest share of the poor quality of oral health is poor oral hygiene. That's why prevention is the most effective tool, provided that the patient is properly educated and motivated to take responsibility for the state of his teeth. From my experience at Dental emergency. We know, that the reality is different. Bachelor thesis is focused on demonstrating the positive impact of dental hygiene. The theoretical part deals with the anatomy and physiology of teeth, briefly describes the most important oral diseases caused by malhygiene. It introduce a possible solution of tooth loss using dental implant and prosthetics. Empirical part is divided into two parts. The first one is focused to demonstrates the positive effect of dental hygiene of patients with established dental implants. This particular group of patients was chosen on purpose, because these patients are properly and repeatedly instructed to care intensely of their implants, because the lifetime of the implant after surgery depends on their care. In a random sample of fifteen patients with dental...
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