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Analytical Features of the Optical Biosensor for Glucose Detection
 Maixnerová, Lucie
The first aim of my work was to determine boundaries of the above-mentioned parameters as maxima and minima of the weight (thickness) of the layer and the quantity of the immobilized enzyme. Glucose oxidase from Aspergilus niger (37.7 KU⋅g−1 solid) immobilized on Sepabeads® (SPB) coated together with the ruthenium oxygen sensitive complex in the organic-inorganic polymer ORMOCER® were used in all experiments. From process parameters maxima and minima concentrations of oxygen and glucose were detected. The second aim was to prepare biosensors with substantially diverse analytical characteristics via combinations of these parameters. The obtained experimental data will be a base for a detailed mathematical model of MATINOES biosensors (outlined in our previous paper1), which we are going to develop in the collaboration with ICT Prague. This model should enable a preparation of MATINOES biosensors with tailor-made analytical features.
Fulltext: content.csg - Download fulltextPDF
Plný tet: SKMBT_C22015061709372 - Download fulltextPDF
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