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Inhibition of the etiological agent of American foulbrood disease using selected essential oils
ZÁLABSKÁ, Adéla
Americal foulbrood disease, caused by etiological agent Paenibacillus larvae is serious disease which affects honey bee that is prohibited to treat with antibiotic in the Czech Republic. If the disease occurs in hive, it is a legal requirement to burn the hive. Essential oils have antimicrobial aktivity and their use can help fighting this dangerous disease. The aim of the bachelor thesis was to verify the bactericidal effect of selected essential oils on the causative agent of the americal foulbrood disease P. Larvae. Another stated aim was to determine the minimum inhibitory and bactericidal concentrations of the selected essential oils. A total of 11 essential oils were used and evaluated both alone and in combination with other essential oils. The evaluation was carried oud on microtiter plates using spectophotometer and on agar medium in petri dishes. The most effective essential oil was garlic oil (Allium sativum bulb oil), which had minimum and bactericidal concentration values of 32 ?l/mg. Other very effective plant essential oils include cinnamon essential oil (Cinnamomum zeylanicum bark oil). The least effective essential oils are carrot oil (Daucus carota sativa seed oil), thyme oils (Thymus serpyllum oil, Thymus vulgaris flower/leaf oil) or oregano oil (Origanum vulgare).

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