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Jak rostliny vnímají chlad: omická analýza
 Karšulinová, Daniela
Plants of temperate zones have developed a sophisticated mechanism that helps them cope with freezing conditions. This process is called cold acclimation. From an agronomical point of view, comprehending this process is necessary to ensure crop yield and quality. This thesis entitled: "Jak rostliny vnímají chlad: omická analýza" describes the molecular mechanism behind the cold acclimation process, specifically the role of light and selected photoreceptors in this process. In the experimental part of the thesis, a mutant line (UVR8 line12-monomer) of model organism Arabidopsis thaliana, which has a partially activated signaling of the UV-B, and the control line (Ws-4) were used. These lines were exposed to cold and UV-B radiation to further study the interaction of the cold signaling pathway and UV-B radiation signaling. LC-MS profiling identified more than 6000 proteins, of which 3472 significantly changed their abundance. Moreover, lipidome analysis was performed using TLC Proteomic analysis revealed an increased abundance of proteins involved in biosynthesis of jasmonic acid. Based on this, a validation experiment with exogenous application of methyl jasmonic acid was performed.
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