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Toxicity of selected nitramines
Papírek, Jan ; Fojt, Pavel (referee) ; Bednařík, Karel (advisor)
This Bachelor Thesis is focused on determination of toxicity of selected nitramines and nitrosoamines, which had to be synthesized soon. Out of total 10 prepared N-nitro and N- nitrosocompounds 4 were selected for testing their toxicity in test using Duckweed (Lemna Minor). Factor IC50 was determined for two compounds 3,7-dinitro-1,3,5,7-tetraazabicyclo [3.3.1]- nonane (DPT) and 3,7-dinitroso-1,3,5,7-tetraazabyciclo [3.3.1] nonane (DNPT), unfortunately factor for other compounds failed to determine, because conditions of test validity hadn’t been kept. In the end of the thesis is compared toxicity of N-nitro and N-nitrosocompounds based on results and observations.
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Drobnička – vodní rostlina jako nový modelový organismus pro studium signálních drah a biotechnologie
Matysková, Tereza
Wolffia spp. are aquatic plants with extremely reduced morphology and rapid reproduction cycle with the shortest recorded doubling time of under 30 hours. These smallest known flowering plants are thus excellent for biotechnology and studying molecular mechanisms underlying growth and development. The theoretical part of this thesis reviews Wolffia spp. and discusses prospective future applications. The experimental part is dedicated to the characterization of W. australiana proteome, employing two complementary strategies for proteome fractionation using an off-line C18 peptides fractionation and an online ion mobility-based fractionation technique. That part is followed by a proteome analysis of W. australiana grown in nutrient-rich media. Finally, results of proteome and metabolome profiling of W. australiana in response to cadmium ions are presented.
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Toxicity of selected nitramines
Papírek, Jan ; Fojt, Pavel (referee) ; Bednařík, Karel (advisor)
This Bachelor Thesis is focused on determination of toxicity of selected nitramines and nitrosoamines, which had to be synthesized soon. Out of total 10 prepared N-nitro and N- nitrosocompounds 4 were selected for testing their toxicity in test using Duckweed (Lemna Minor). Factor IC50 was determined for two compounds 3,7-dinitro-1,3,5,7-tetraazabicyclo [3.3.1]- nonane (DPT) and 3,7-dinitroso-1,3,5,7-tetraazabyciclo [3.3.1] nonane (DNPT), unfortunately factor for other compounds failed to determine, because conditions of test validity hadn’t been kept. In the end of the thesis is compared toxicity of N-nitro and N-nitrosocompounds based on results and observations.
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