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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Detection viability of bacteria population using flow cytometry
 TŮMOVÁ, Lucie
This work deals with the detection of viability of Xanthomonas euvesicatoria using a flow cytometer. The center of this work was tp optimize the method and the treatment of bacterial cultures with high temperature at given time intervals. The heat-treated cultures were dyed with an intercalating dye called propidium iodide which marks the permeabilized membranes of the cells and thus provides information on the number of dead (apoptotic) cells. The evaluation of the results confirmed the assumption that the number of surviving cells would decrease with the increasing treatment time. A temperature of 90 ° C was used, which according to other works, seems to be the most evident for the Xanthomonas genus. Flow cytometry has proven to be a simple, fast and efficient method for the viability detection of Xanthomonas euvesicatoria.
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Detection of viability of \kur{Pseudomonas syringae} pv. \kur{oryzae} using flow cytometry
 KOLÁŘOVÁ, Johana
The viability of Pseudomonas syringae pv oryzae was analyzed by flow cytometry and intercalating dye propidium iodide in this thesis. The treatment of bacterial cultures with high temperatures in time series was the basis of this thesis. Statistically evaluation of the data obtained acknowledged the tendency of decrease of number of surviving cells in the population, depending on the growing period of treatment.
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