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Stability of Soil Organic Matter
Heldesová, Michaela ; Doskočil, Leoš (referee) ; Kalina, Michal (advisor)
This bachelor thesis summarizes available information about current research of soil organic matter stability and it divides them into two logical units. The first part includes environmental, physical and chemical factors, including human activities, which affect the stability or decomposition of soil organic matter. The methods used for the study of organic matter in the soil are summarized in the second part of this thesis. There the attention is paid to the accumulation of organic matter in specific pools, the ways of extraction of organic compounds and the use of quantitative and qualitative analytical methods to describe various characteristic of organic matter.
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Metabolomics in the study of microbiome
Nazmutdinova, Anastasiia ; Kuzma, Marek (advisor) ; Cahová, Monika (referee)
Inflammatory bowel disease is a serious condition with an incomplete etiology and pathogenesis. In this thesis, a mouse model of sodium dextran sulfate-induced inflammation was used to study different changes in the metabolism of germ-free and conventionally raised mice due to the development of the inflammatory process. NMR metabolomics of fecal, urine and serum samples, combined with uni- and multivariate statistical analysis, were used to characterize the changes. It was shown that the metabolic signature differs between germ-free and conventional mice. In germ-free mice, significant amounts of carbohydrates were found in feces. Their levels decreased during inflammation as they were excreted in urine. In contrast to conventional mice, germ-free mice also excreted large amounts of amino acids in feces during the developing inflammation. Disorders of sugar and protein metabolism found in germ-free mice indicate severe malnutrition caused by inflammation. The results show that the presence of a microbiome represents a protective mechanism against significant disruption in the body. A stability study of fecal extracts of healthy conventionally colonized mice confirmed that none of the identified and quantified metabolites showed significant systemic changes in several consecutively collected...
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Stability of Soil Organic Matter
Heldesová, Michaela ; Doskočil, Leoš (referee) ; Kalina, Michal (advisor)
This bachelor thesis summarizes available information about current research of soil organic matter stability and it divides them into two logical units. The first part includes environmental, physical and chemical factors, including human activities, which affect the stability or decomposition of soil organic matter. The methods used for the study of organic matter in the soil are summarized in the second part of this thesis. There the attention is paid to the accumulation of organic matter in specific pools, the ways of extraction of organic compounds and the use of quantitative and qualitative analytical methods to describe various characteristic of organic matter.
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