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Molecular characterisation of microsporidia causing nosematosis in bees
HANUŠOVÁ, Adéla
Microsporidia of the genera Nosema apis and Nosema ceranae cause bee intestinal disease, which is widespread throughout the world, including the Czech Republic. The aim of my bachelor thesis was to evaluate the occurrence of Nosema in various places in the Czech Republic. The literature contains only a limited amount of information about nosematosis caused by both microsporidia. In total, over 135 samples were taken from 27 sites. Samples were taken from November 2019 to January 2020. Sampling took place in the vicinity of large cities, in their vicinity, where the landscape was cultivated and in places with little agricultural impact. After analysis of the obtained samples by PCR, the vast majority of microsporidia of Nosema cerenae was present, while Nosema apis was nowhere.
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Vyšetření včelstev na nosematózu
BRABENEC, Jiří
Nosematosis belongs to the intestinal disease of bees, which is widely spread both in the world and in the Czech Republic. It is caused by Nosema apis and Nosema ceranae. The aim of this diploma thesis was to evaluate the occurrence of the Nosema dispute in the territory of ČSV Bystřice, to describe species variability and to assess the effect of the season on the occurrence of Nosema apis and Nosema ceranae. To detect infection of colonies, 5 bees from selected hives were examined under the microscope. In positive specimens on nosematosis, the species of Nosema spore was molecularly examined. In total, 93 samples from 15 breeders were examined. Samples were taken in the spring and summer of 2017. The sampling took place both for malachists and for farmers with more than 150 bee colonies. During the summer sampling more positive bees were found than in spring sampling. Beekeepers were further assessed by the number of hives. Following the analysis of the samples obtained using the PCR method, Nosema ceranae disputes were present in the vast majority of colonies.
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Intestinal disorder of bees
BRABENEC, Jiří
The aim of this thesis was to describe the most common intestinal disease of bees. Constipation, dysentery and nosematóza belong among them. This diseases are caused by Nosema apis and Nosema ceranae. In recent years, Nosema apis is suppressed by Nosema ceranae. The difference between these types is recognizable only under a microscope. Symptom appear after outbreak of desease and it is very dangerous. In Czech Republic there is not allow any direct therapeutic medicine. Therefore, we should emphasize the importance of prevention and thorough disinfection of beekeeping equipment.
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