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National Repository of Grey Literature 1 records found  Search took 0.01 seconds. 
Využití bioluminiscenčních, histologických a imunohistochemických technik při studiu lokalizace produktů genové exprese v tkáních hmyzu
 PAUCHOVÁ, Lucie
The genes of enzymes catalyzing the bioluminescence reactions are commonly used as reporter genes in molecular biology. These markers are applied especially, in the studies of daily oscillation of gene expression products involved in the circadian clock. Here is reported an improved technique for monitoring the clock gene expression on the single neuron level in the brain of Drosophila melanogaster by using the bioluminescence marker. A part from that another study is focused on localizing a newly identified neuropeptide TEFLa in different developmental stages of firebug Pyrrhocoris apterus. This neuropeptide was detected in the brain of larvae and adults, in the posterior region of the midgut of adults and in the gonades of larvae. Beside that, it was found in the region of amnion closure in embryos. Synthesis of TEFLa in the adults' gut is controlled by the circadian rhythms. Identification of TEFLa distribution helps to uncover the function of this neuropeptide.
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