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Účinnost mykoparazitických a entomopatogenních hub proti houbovým původcům onemocnění rostlin
SINKALA, Natasha
Entomopathogenic and mycoparasitic fungi have the potential to be used both locally and internationally as an effective and environmentally friendly strategy for the man-agement of pests and plant diseases caused by fungal pathogens. In this bachelor thesis, the efficacy of beneficial fungi was tested under laboratory conditions. The methodology involved isolation and description of useful fungal species and subse-quent testing of useful fungi with potential mycoparasitic and antagonistic effects. The efficacy of the fungi was tested on pathogens that were mainly isolated from seeds or diseased legume plants. The experiments revealed that all Trichoderma strains isolated from soil of the Czech Republic showed mycoparasitic effect on se-lected pathogens. However, the efficacy of the strains depended on the type of fun-gal pathogen. Spore production of Trichoderma strains was also evaluated both in the control variant as well as the mutual interactions with pathogens. The results again showed differences in spore production among the Trichoderma strains. The myco-parasitic effect of the entomopathogenic fungi Isaria fumosorosea and Akanthomyces attenuatus against the pathogen Botrytis cinerea was also evaluated. The results re-vealed that although I. fumosorosea and A. attenuatus showed a significant mycopar-asitic effect against Botrytis cinerea, the mycoparasitic fungus T. virens showed the highest efficacy against the pathogen. The findings indicated that both mycoparasitic and entomopathogenic fungi are sustainable alternatives to artificial pesticides in the fight against fungal agents of plant diseases.
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Monitoring of natural occurence of mycoparasitic and antagonistic fungi in soil in the region of South Bohemia
BÍLKOVÁ, Jana
The thesis is focused on monitoring of natural occurrence of mycoparasitic fungi in arable soils. Study was aimed to isolate those fungi from selected plots with conventional and organic farming systems of the region of South Bohemia. Four isolated method were used for isolation of mycoparasitic fungi from soil.Among all, species of genus of Trichoderma, Trichoderma virens, Clonostachys rosea f. catenulata and Lecanicillium muscarium were the most frequently present and isolated species.The radial growth of strains and their production characteristic was evaluated. The efficacy of Trichoderma strains against phytopathogenic fungi (S. sclerotiorum, Rhizoctonia solani, Botrytis cinerea a Fusarium solani) was evaluated.
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Induction of suppressive soil by introduction of mycoparasitic fungi against important plant pathogens
STREJČKOVÁ, Monika
This M. Sc. thesis is based on using of mycoparasitic fungi Trichoderma virens, Clonostachys rosea f. catenulata in biological control against phytopathogenic fungi Sclerotinia sclerotiorum, Rhizoctonia solani, Botrytis cinerea and Fusarium solani. The efficacy of mycoparasitic fungi against pathogens was evaluated in dual cultural tests. The strains of T. virens and C. rosea f. catenulata isolated from soils in the Czech Republic were tested in the experiment. Reference strain was GL 21 fungus T. virens reisolated from commercially available bio-preparation SoilGard and strain C. rosea f. catenulata reisolated from Prestop Mix. All the strains were tested for biological and production properties. All strains are able to colonize the substrate and to suppress the growth and development of pathogens. Strain GL 21 of T. virens was used for seed coating of variety Scirocco in combination with products Guar gum and Carboxymethyl cellulose, which served as a carrier for stick on conidia. After 3 days, the effect of fungus T. virens was evaluated on energy of germination, development of roots of grain. The grain health was determined after 7 days. The fungus T. virens has a positive effect on the grains germination and grain health. During the vegetation the influence of seed coating by T. virens was observed on growth and development of spring wheat. The parameters such as number of plants per m2, tiller numbers, plants health, stand height, number of grains in the spike and thousand grain weight (TGW) were evaluated. During the vegetation the fungus T. virens has positive effect on the plant height.
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