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Optimization of optical tweezers for multimodal holographic microscope
Vaverka, Jan ; Antoš, Martin (referee) ; Dostál, Zbyněk (advisor)
This bachelor's thesis has described the principle of operation of interference microscopes with closer analysis of multimodal holograpfic microscope (MHM), which has been constructed at the Institut of Physical Engeneering (IPE) of Brno University of Technology. It has also analysed principles of optical trapping and has closely descirebed force effects on the trapped object with characteristic lenght bigger than wavelenght of trapping beam. It has designed optimization of existing optical tweezers module so that the module is connectable to the prototype of MHM, located at IPE. Simulation and optimization of the optical assembly of module have been performed, the mechanical design of module with the drawings has been designed. The module has been entered into production.
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Fluorescence imaging techniques in multimodal holographic microscope
Vašíček, David ; Procházková, Jana (referee) ; Čolláková, Jana (advisor)
The diploma thesis deals with the registration of images taken with the multimodal holographic microscope (MHM). The summary covers the fluorescent and holographic microscopy, and the multimodal holographic microscope combining both these microscopy types. Every pair of the images needs to be aligned in order to gain new information by combining both image types. The thesis contains an algorithm that registers images by phase correlation as well as a process created in MATLAB in accordance with the algorithm. The most important procedure parameters’ influence on the registration success is described and the results are annotated.
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Cell tracking in images from holographic microscope
Vičar, Tomáš ; Odstrčilík, Jan (referee) ; Kolář, Radim (advisor)
This thesis focuses on cell tracking in image sequences acquired using a multimodal holographic microscope (MHM). The principles of holographic microscopy are described together with the application in cells acquisition. The main part of the thesis describes a complete approach for segmentation and tracking of single cells in acquired in long-term sequences. The approach is designed based on parametric active contour models with specific modifications to achieve reasonable precision and robustness. The implemented method is described in detail, including the evaluation and demonstration of results.
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Observation of external environment influence on living cells with holographic microscope
Kovářová, Klára ; Tolde,, Ondřej (referee) ; Křížová, Aneta (advisor)
Subject of this master's thesis is the observation of influence of external environment on the living cells with the use of multimodal holographic microscope. The theoretical part is summarising the development of the holographic microscopy at IPE FME BUT. The theoretical part also describes multimodal holographic microscope, which allows non-invasive observing of living cells. The thesis also covers construction of the microscope, basic working instructions and the hologram processing method. The main subject of the thesis is the research on the topic of chemotaxis and osmotic processes in the cells. Experiments were designed for the purpose of this thesis to cover topics mentioned above. The experimental part of the thesis deals with cultivation of the cells, preparation of the sample and observation chambers and processing of the data. This part later focuses directly on the laboratory measurements. In all experiments, cells K2 (full name LW13K2) were observed.
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Cell tracking in images from holographic microscope
Vičar, Tomáš ; Odstrčilík, Jan (referee) ; Kolář, Radim (advisor)
This thesis focuses on cell tracking in image sequences acquired using a multimodal holographic microscope (MHM). The principles of holographic microscopy are described together with the application in cells acquisition. The main part of the thesis describes a complete approach for segmentation and tracking of single cells in acquired in long-term sequences. The approach is designed based on parametric active contour models with specific modifications to achieve reasonable precision and robustness. The implemented method is described in detail, including the evaluation and demonstration of results.
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Observation of external environment influence on living cells with holographic microscope
Kovářová, Klára ; Tolde,, Ondřej (referee) ; Křížová, Aneta (advisor)
Subject of this master's thesis is the observation of influence of external environment on the living cells with the use of multimodal holographic microscope. The theoretical part is summarising the development of the holographic microscopy at IPE FME BUT. The theoretical part also describes multimodal holographic microscope, which allows non-invasive observing of living cells. The thesis also covers construction of the microscope, basic working instructions and the hologram processing method. The main subject of the thesis is the research on the topic of chemotaxis and osmotic processes in the cells. Experiments were designed for the purpose of this thesis to cover topics mentioned above. The experimental part of the thesis deals with cultivation of the cells, preparation of the sample and observation chambers and processing of the data. This part later focuses directly on the laboratory measurements. In all experiments, cells K2 (full name LW13K2) were observed.
|
|
|
Fluorescence imaging techniques in multimodal holographic microscope
Vašíček, David ; Procházková, Jana (referee) ; Čolláková, Jana (advisor)
The diploma thesis deals with the registration of images taken with the multimodal holographic microscope (MHM). The summary covers the fluorescent and holographic microscopy, and the multimodal holographic microscope combining both these microscopy types. Every pair of the images needs to be aligned in order to gain new information by combining both image types. The thesis contains an algorithm that registers images by phase correlation as well as a process created in MATLAB in accordance with the algorithm. The most important procedure parameters’ influence on the registration success is described and the results are annotated.
|
|
|
Optimization of optical tweezers for multimodal holographic microscope
Vaverka, Jan ; Antoš, Martin (referee) ; Dostál, Zbyněk (advisor)
This bachelor's thesis has described the principle of operation of interference microscopes with closer analysis of multimodal holograpfic microscope (MHM), which has been constructed at the Institut of Physical Engeneering (IPE) of Brno University of Technology. It has also analysed principles of optical trapping and has closely descirebed force effects on the trapped object with characteristic lenght bigger than wavelenght of trapping beam. It has designed optimization of existing optical tweezers module so that the module is connectable to the prototype of MHM, located at IPE. Simulation and optimization of the optical assembly of module have been performed, the mechanical design of module with the drawings has been designed. The module has been entered into production.
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