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Phylogeography and genetic diversity of the populations of Gobius niger (Gobiiformes: Gobiidae)
Hradečný, Jakub ; Vukićová, Jasna (advisor) ; Perdices, Anabel (referee)
Gobies (Gobiiformes: Gobiidae) are the most speciose fish family and the most abundant fish group of the European seas. Yet, the population genetic diversity has been studied in only a few European goby species. Although the ocean is seemingly a continuous habitat, there are barriers to gene flow in marine environment in form of e.g. straits or marine currents. The black goby (Gobius niger) is a demersal marine fish with planktonic larvae, inhabiting the coastal waters of Europe, north Africa and the Mediterranean and Black Sea coast of the Middle East. The current work examined individuals from 28 localities across the distribution range of the species in order to study the population genetic diversity. The analyses of two mitochondrial markers (cytochrome b and D loop) revealed a complex structuring of the population and a pronounced intraspecific diversity (maximum uncorrected p-distance 6.1 %). The population is divided into four very diverse haplotype groups, with unexpected geographic pattern. While the population inhabiting the Atlantic Ocean and the North Sea is generally genetically homogeneous, with all examined individuals bearing haplotypes belonging to a single haplotype group, the Mediterranean population is very complex, displaying a presence of four haplotype groups, some of which...
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Phylogenetic relationships within the Gobius-lineage (Gobiidae)
Slámová, Tereza ; Vukićová, Jasna (advisor) ; Tougard, Christelle (referee)
Gobiidae is one of the largest families of teleost fishes with nearly 2000 species currently recognized. They have a worldwide distribution with exception of Arctic and Antarctic areas, inhabiting marine, brackish and freshwaters. Mostly, they are small-sized and live inconspicuously on the bottom. Their phylogeny has been studied only partially. In Europe, three independent lineages of gobies exist (Gobius-, Aphia- and Pomatoschistus-lineage), most of the species of these lineages are marine. In this work, I performed a multilocus study of the Gobius-lineage encompassing the majority of the species. Mitochondrial (cytochrome b and cytochrome c oxidase I) as well as nuclear (rhodopsin and recombination activating gene) markers were used. 480 individuals of 30 species were analyzed in the laboratory and sequences of further 25 - 32 species were downloaded from the Genbank and added to a dataset of each marker according to availability. Mitochondrial markers were more informative than the nuclear ones. The usefulness of cytochrome c oxidase I for studying phylogenetic relationships of gobies was compared with cytochrome b. Cytochrome c oxidase I showed to be useful for identification of the species, but has some limitations in resolving deeper phylogenetic relationships in gobies. Cytochrome b showed...
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