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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Genotyping of locu CSN1S1 and association analysis
 BRABEC, Václav
The first aim of this work was to genotype polymorphism locus CSN1S1. This gene contains a transition in the exon XVII. This transition changes glycine to glutamine in the position 192 of the final protein chain. This substitution creates two polymorphism variants of this gene: variant B and C. In our study, two genotypes BB and BC were observed. The second aim of this work was evaluation of the relationship among observed genotypes and milk properties and technological milk traits, e.g. curd firmness. The analysis was performed on 234 samples from Holstein Friesian cattle and Czech fleckvieh cattle sampled on farms in the South Bohemia. Genotyping was performed using molecular biology techniques PCR and RFLP. Restriction endonuclease HpHI was used for RFLP. Fragments were visualized on agarose gel. In our monitored population the frequencies of the genotypes BB and BC were 0,888 and 0,112. Association analysis was performed using SAS analytics software. Provable influence of the CSN1S1 locus polymorphism on studied traits was not observed.
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Polymorfismus kandidátního lokusu a jeho vliv na technologickou jakost mléka
 ŠVARCOVÁ, Monika
This thesis was developed to study the influence of polymorphic variants of the milk protein locus betacasein (BCN) on the production and technological quality of the milk. The genotypes of -casein (CSN2 gene) were determined by the PCR-RFLP analysis. The determination of genotypes AA, AB and BB was made in 731 dairy cows. The results of genotype frequencies showed that genotype BB represented roughly five times more frequently than the heterozygotes AB and thirty times more frequently than AA homozygotes in the population under study. The effect of the BCN genotypes on the production and composition of milk was analyzed in 418 samples. No statistically significant effect of genotypes or alleles was found in this section. Subsequent analysis of the influence of genotypes and allele on the coagulation and the determination of titratable ability of the milk was performed on 119 samples. The results did not confirm a statistically significant effect on milk coagulation. The yogurt test showed genotypic effects. This could mean that genotype AA is associated with higher milk acidity and AB genotype with lower. However, the results could be affected by lower frequency of the population and an uneven frequency of genotypes in the population surveyed.
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