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Analysis of gonosomal mosaic using FISH method
ŠUTKOVÁ, Jana
In my bachelor thesis I dealt with the analysis of gonosomal mosaics. Mosaicism can be described as a condition in which two or more distinct cell lines originating from a single zygote are found in one organism. In women, the most common mosaic forms are Turner syndrome and triple X syndrome or superfemale. In the male population, Klinefelter syndrome and XYY syndrome (supermale) are most commonly detected. Mosaicism can be investigated by fluorescence in situ hybridization. In the theoretical part of my bachelor's thesis I focused on congenital chromosomal mosaicism, especially on its variants such as Klinefelter syndrome, Turner syndrome, triple X and XYY syndrome. Furthermore, in this part I dealt with fluorescence in situ hybridization. I described fluorescence per se and then its application in clinical practice, where several types of fluorescence in situ hybridization are used. In the practical part I examined the number of gonosomal chromosomes using fluorescence in situ hybridization. I prepared this part in the laboratory of the Faculty of Health and Social Sciences of the University of South Bohemia in České Budějovice. I examined 40 anonymized samples from 20 probands. Capillary blood samples and buccal swabs were taken from each of them. After hybridization of samples with centromeric probe for X and Y chromosomes microscopy was performed. In the last part of this bachelor thesis I dealt with the processing of the results obtained by fluorescence microscopy. Thus, a total of 6 cases of mosaicism were detected in women (the percentage of mosaic cells was above the admissible limit). In males there were a total of 2 cases of increased percentage of mosaic cells.
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Evaluation of upper secondary students' microscopy skills
Míková, Zuzana ; Janštová, Vanda (advisor) ; Mourek, Jan (referee)
High-school students are less interested in science then before. Despite importance of science for our life and for description of nature around us, students lose motivation for studying it. The approach of teacher is critical for focusing students' attention. Lessons should be based on linking of the education with daily-life and biology provides plenty of possibilities to achieve the goal, especially employing hand-on education, practical examples and exercises. One of the most effective way to show the beauty and a complexity of organism structure is by using microscopes. Many different techniques have been used to prepare education materials - picture, videos or whole movies from micro-word. Nevertheless, a classical optical microscopy is by far the most common training activity during the practical courses. An evaluation of this education aspects is, therefore, critical for further method development. This diploma thesis is focused on establishing of evaluation techniques and subsequent analysis of the current state of students' microscopy skills. First of all, the elementary set of knowledge and the correct microscopy work-flow were established in collaboration with eleven experienced teachers. Based on these results, two types of surveys were designed. One of them was dedicated to teachers...
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