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Introduction of microbiological diagnostic agents of invasive infections - Bartonella henselae and Bartonella quintana
Nováková, Marie ; Melter, Oto (advisor) ; Chrenková, Vanda (referee)
Bartonellae are agents of infections with varied clinical symptomalogy, including serious, such as endocarditis. Direct detection of Bartonella so far in the Czech Republic and therefore does not need to address this issue. Proper diagnosis of these infections is a prerequisite for targeted antibiotic therapy for patients. The genus Bartonella today are more than 20 kinds of bacteria. They are small Gram-negative rod-shaped bacteria that cause the new emerging infections such as cat scratch disease (CSD), showing the typical regional lymphadenitis, as well as bacillary angiomatosis (BA), whose typical symptoms are skin and subcutaneous nodules (nodules). They also cause a disease called trench fever or South American bartonelosis. In the experimental part of the work was performed genotypic and phenotypic methods for the diagnosis of these bacteria, specifically on two types of Bartonella, Bartonella henselae and Bartonella quintana . The results show that by suitable methods to diagnose Bartonella are amplification genus- specific genes ribC and ftsZ, further embodiment of a method pulsed electrophoresis (PFGE) as typing method and the determination of susceptibility to antibiotics, the most appropriate testing showed E-test. This work was carried out optimization of genotypic and phenotypic...
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Introduction of microbiological diagnostic agents of invasive infections - Bartonella henselae and Bartonella quintana
Nováková, Marie ; Melter, Oto (advisor) ; Chrenková, Vanda (referee)
Bartonellae are agents of infections with varied clinical symptomalogy, including serious, such as endocarditis. Direct detection of Bartonella so far in the Czech Republic and therefore does not need to address this issue. Proper diagnosis of these infections is a prerequisite for targeted antibiotic therapy for patients. The genus Bartonella today are more than 20 kinds of bacteria. They are small Gram-negative rod-shaped bacteria that cause the new emerging infections such as cat scratch disease (CSD), showing the typical regional lymphadenitis, as well as bacillary angiomatosis (BA), whose typical symptoms are skin and subcutaneous nodules (nodules). They also cause a disease called trench fever or South American bartonelosis. In the experimental part of the work was performed genotypic and phenotypic methods for the diagnosis of these bacteria, specifically on two types of Bartonella, Bartonella henselae and Bartonella quintana . The results show that by suitable methods to diagnose Bartonella are amplification genus- specific genes ribC and ftsZ, further embodiment of a method pulsed electrophoresis (PFGE) as typing method and the determination of susceptibility to antibiotics, the most appropriate testing showed E-test. This work was carried out optimization of genotypic and phenotypic...
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The most common bacterial originators of intestinal diseases in adults in the Czech republic
KOPECKÁ, Kristýna
My bachelor thesis focuses on the most frequent bacterial causes of intestinal diseases in adults in the Czech Republic. I deal with bacterial intestinal pathogens of Salmonella, Shigella, Yersinia, Campylobacter genus. The theoretical part characterises the selected intestinal bacterial pathogens and their microbial diagnostics. In the present time of progress various new and modern methods and instruments keep appearing to accelerate and improve diagnostics. But I focus exclusively on methods used as a routine in clinical microbiological laboratories. These methods include cultivation of microbes, biochemical specification and sensitivity tests. The proof by cultivation is a standard procedure. Biochemical tests are used for microbe identification. Antigen analysis is used for further specification of bacteria. Specification of sensitivity to antibiotics for correct therapy is an integral part of this method. The practical part describes laboratory diagnostics of bacterial intestinal pathogens from stool swab used by the accredited microbial laboratory of RNDr. Alena Veselská in Ústí nad Labem, which I performed in practice while on scholarship there. The results summarise laboratory results from the period October 2012 to March 2013. positive results are compared to the data obtained from Epidate of the National Healthcare Institute in Prague included in the report of the Epidemiology and Microbiology Centre. I have further processed finds of intestinal pathogens in the period 2003 to 2012 published in CEM reports. The results show that the most frequently reported bacterial intestinal pathogen in the Czech Republic in the period 2003 to 2006 was Salmonella, followed by Campylobacter. Situation changed in 2007, when diagnosed Campylobacter moved to the top rank in the Czech Republic. In contrast to the two abovementioned bacteria finds of Shigella are relatively scarce.
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