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Posouzení účinnosti stromových mikroinjektáží jako metody aplikace navnaděných stojících otrávených lapáků na LZ Boubín
Řezník, Pavel
The topis of this thesis was to evaluate the effectiveness of tree microinjection as a method of forest protection management. The evaluated data were following:maternal and larval corridors lengths of Ips typographus and mortality of I. typographus on treated and control trees in photoeleclectors . Data collected during the field experiments were statistically tested for the presence of differences using non-parametric analysis of variance (Kruskal-Wallis, resp. Dunn's t-test). The secondary aim of this thesis was to assess the use of the method as a poisoned standing baited trap in forestry practice in the Klet' forest area belonging to the Boubín Forestry Plant (Lesy České republiky, s. p.). Both measurements of the length of maternity and larval tunnels and the amount of beetles captured from photoeleclectors showed significant effectivity on reducing of I. typographus development and increased mortality pressure for spruce bark beetle adults.
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Zhodnocení efektivity stromových mikroinjektáží jako metody tvorby navnaděných stojících otrávených lapáků na lýkožrouta smrkového (Ips typographus Linnaeus, 1758) na lesních pozemcích Města Poličky
Háp, Matěj
Many technologies are currently used in practice for pest management and control. One technology that is becoming more widely used is the microinjection method. This method is the focus of this thesis, which specializes in the use of microinjection as a defensive method against the European spruce bark beetle (Ips typographus (Karst.) L.). The investigation took place in 2022, when 25 trees were injected at 5 sites before the start of spring swarming. In addition, 5 control trees were selected at each site and I. typographus was baited with a synthetic pheromone. The effectiveness of this method was then evaluated by comparing the lengths of the mother galleries and larval galleries on the debarked bark strips and by means of a photo-eclector experiment. The data were then statistically evaluated using the Kruskal-Wallis test, which showed that bark beetles on trees treated with injection had significantly shorter mother and larval galleries. The photo-eclector survey showed that the number of I. typographus from logs that came from injection-treated trees accounted for only 10.88% of the total number of imagoes captured. This value demonstrates that microinjection is not only an effective method against leaf miners and skeletonizing insects on deciduous trees, but its application can also be in the management of bark beetles on spruce.
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Role genu Socs3 v preimplantačním vývoji myších embryí
TOMÁNKOVÁ, Jana
The Socs3 gene is important for the postimplantation development of mouse embryos, but not much is known about its effect on preimplantation development. The aim of this study was to determine the effect of Socs3 gene in preimplantation development of mouse embryos, specifically on proliferation at E3.5 timepoint, at the time of seggregation of cell lineages trofoectoderm and inner cell mass.
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Making Transgenic \kur{C. elegans} with Polycistronic mCherry Vector
FARKA, Dominik
Creation of transgenic animals has become a popular method to analyse gene function. In the nematode Ceanorhabditis elegans transformation is widely used and can be achieved by microinjection. For functional analyses, transgene constructs typically contain a promoter driving the expression of the protein of interest that is fused to a fluorescent protein. However, as this fusion of proteins can lead to misfolding of the protein of interest and may not reflect proper function, a modification of the expression vector has been developed; introducing a short sequence of non-coding DNA in-between the sequences of the two proteins and making the construct compatible with a polycistronic operon system. In this study, four different polycistronic constructs were introduced into C. elegans by means of microinjection in order to provide new tools for the analyses of gene function. Tissue specific promoters wrt-2 (seam cells), grl-21 (hyp7), and egl-17 (vulval precursor cells) were used to over-express either NHR-25 or SMO-1 in the corresponding tissues and the expression was visualized by independently translated mCherry red fluorescent. 10 independent transformed C. elegans strains were established and corresponding tissue-specific promoter activities were confirmed. Furthermore, in some cases, ectopic behaviour was observed e.g. ectopic mCherry expression in different tissues or specific cell differentiation defects that was most likely caused by the overexpression of NHR-25 or SMO-1. This study was the first case in our laboratory to generate transformed C. elegans utilizing the polycistronic mCherry vector system. New genetic tools were introduced in the laboratory useful for further analyses of gene function.
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