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Preparation and characterization of surface-modified nanoliposomes using click chemistry techniques
Frydrychová, Aneta ; Bartoš,, Milan (referee) ; Turánek, Jaroslav (advisor)
Over the past decades the liposomes have been intensively studied for their unique properties which predispose them for use as drug delivery systems or for constructions of vaccines. This diploma thesis provides an overview of their most important properties, preparation options and their surface modification. The aim of this thesis is thus a preparation and characterization of the nanoliposomes and their surface modification. The liposomes were prepared by lipid film hydration method and mannan polysacharide was used for surface modification. Due to the use of a lipid with an N-oxy group, the modification was carried out via an oxime ligation via click chemistry. Nanoliposomes were characterized by series of physicochemical methods such as TEM, DLS, FT-IR or nano-flow cytometry. Part of the thesis is a study of the interactions of liposomes accomplished on selected cell lines to verify whether they stimulate immune response pathways. Its results confirmed activation of the NLRP3 inflammasome leading to the production of pro-inflammatory cytokines (IL-1). Thus, these polymer coated nanoliposomes are potentially useful as vaccine adjuvants.
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Preparation and characterization of surface-modified nanoliposomes using click chemistry techniques
Frydrychová, Aneta ; Bartoš,, Milan (referee) ; Turánek, Jaroslav (advisor)
Over the past decades the liposomes have been intensively studied for their unique properties which predispose them for use as drug delivery systems or for constructions of vaccines. This diploma thesis provides an overview of their most important properties, preparation options and their surface modification. The aim of this thesis is thus a preparation and characterization of the nanoliposomes and their surface modification. The liposomes were prepared by lipid film hydration method and mannan polysacharide was used for surface modification. Due to the use of a lipid with an N-oxy group, the modification was carried out via an oxime ligation via click chemistry. Nanoliposomes were characterized by series of physicochemical methods such as TEM, DLS, FT-IR or nano-flow cytometry. Part of the thesis is a study of the interactions of liposomes accomplished on selected cell lines to verify whether they stimulate immune response pathways. Its results confirmed activation of the NLRP3 inflammasome leading to the production of pro-inflammatory cytokines (IL-1). Thus, these polymer coated nanoliposomes are potentially useful as vaccine adjuvants.
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Functionalized hybrid polymer structures for biomedical applications
Rabyk, Mariia ; Štěpánek, Petr (advisor) ; Sedláková, Zdeňka (referee) ; Kotek, Jan (referee)
This doctoral thesis is dedicated to the synthesis and characterization of novel functionalized hybrid structures for biomedical purposes. Systems reported in this work can be subdivided into the two main groups: natural-based materials and synthetic amphiphilic block copolymers. Both groups were studied as perspective theranostic agents for medical applications. In the first group, natural polysaccharides glycogen and mannan were selected as starting materials for preparation of novel nanoconjugates that possess ability for multimodal detection in vivo. Because grafting of natural macromolecules with synthetic polymers generally slows down the biodegradation rate, both polysaccharides were modified in two different ways to form nanoprobes with or without poly(2-methyl-2-oxazoline)s chains. The prepared nanoconjugates were functionalized with N-hydroxysuccinimide-activated fluorescence and magnetic resonance imaging labels. The resulting materials were tested both in vitro and in vivo and were shown to be completely biocompatible, biodegradable and exhibit some extra benefits in terms of their practical usage in biomedicine. Glycogen was functionalized with allyl and propargyl groups with following freeze-drying from aqueous solutions to form nano- and microfibrous materials. The presence of both...
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