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Barcoding společenstva makrozoobentosu Křesánovského potoka
PEJCHAROVÁ, Eliška
Molecular genetic methods, including barcoding, have been increasingly utilized in recent years for the analysis of communities of macrozoobenthos and many other organisms. Morphological identification of precise species of mayflies, caddisflies, or stoneflies in their larval stage is demanding and requires a significant amount of time and expertise. Barcoding analysis can help to classify disputed species more accurately. The aim of this thesis is to use the barcoding method to determine selected representatives of macrozoobenthos and verify the morphological determination of macrozoobenthos representatives. The studied locality is Křesánovský Stream in southern Bohemia. DNA was extracted from the collected samples and using mitochondrial gene CO1 and primer pairs LCO1490, HCO2198, jgLOC1490, and jgHCO2198, sequences were created and tested in the BLAST program. The most similar sequences from publicly available databases were obtained, compared to sequences from this thesis and the exact species were subsequently determined. A total of 57 samples were studied, from which 29 species were morphologically identified (six species of mayflies, eight species of caddisflies, seven species of stoneflies, three representatives of beetles, four species of dipterans, and one specimen of Gammarus fossarum), and 30 species were identified through barcoding (11 species of mayflies, eight species of caddisflies, five species of stoneflies, two species of dipterans, three species of beetles, and one species of fleas). Morphological determination was successful in 63% of cases. The success rate was 74% for mayflies, 67% for caddisflies, 33% for stoneflies, and 25% for dipterans. The specimen of Gammarus fossarum was morphologically identified correctly. Barcoding appears to be a suitable method for studying macrozoobenthos and an excellent complement to morphological analysis of samples. However, it has its limitations, and it is not appropriate to consider it as the sole method for determining macrozoobenthos samples.
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The load of sediments and bentic organisms with trace metals in urban stream Botič
Hanzlíková, Lenka ; Dočkalová, Kateřina (advisor) ; Stuchlík, Evžen (referee)
Trace element bioaccumulation in benthic organisms is important part of aquatic environment pollution research, since water and sediment analysis itselfs do not provide significant information about bioavailability of contaminants and the results mostly shows just current pollution at the time of sampling. This study focuses on urban streams contamination which are currently significant source of trace elements. Botic stream was chosen as exemplary, because it is affected by many combined sewer system overflows which are sources of contamination in times of heavy rain and flood. Load several sampling took place on 10 stream sites during the year of 2012. This thesis deals with 11 trace elements concretelly Cd, Co, Cr, Cu, Hg, Ni, Zn, As, Fe, Pb and Al. Trace elements were assessed in both benthic organisms and sediment. As well taxons were selected from benthic organisms, which are widely found at any time of the reference year: caddisflies of family Hydropsychidae, leeches Erpobdella sp. and mayflies of Baetidae family. Sediment analysis included sequential extraction which divided trace elements into 4 fractions according to mobility. Based on the results, bioavailability was assessed on observed elements and correlation between concentration in bethos and sediment was tested. Finally, impact of...
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The use of artificial substrates for evaluation of effluent water quality from organic and conventional carp ponds
GRACÍK, Jan
The objective of this diploma thesis was to evaluate the quality of the water after flow through the ponds with conventional (Blatensko, Czech Republic) and organic (Waldviertel, Austria) technologies using the colonization of artificial substrates by macrozoobenthos (MZB) to asses the differences in the influence of different managements of pond farming (organic vs. traditional) on water quality. Samplings were performed in monthly intervals (AprilSeptember 2016) at nine inflow and outflow profiles of four ponds. Samples of MZB were processed in the laboratory. Evaluation of MZB was supplemented by in-situ monitoring of basic parameters of the aquatic environment. The resulting values of saprobic index (SI) match the water quality in outlets in the range valid for the beta to alpha mesosabrobity (SI 2.302.62). In the inlets to the Skaličný and Haslauerteich ponds, where the SI value corresponded the betamesosabrobity (SI 2.482.49), the discharged water quality in the outlet was insignificantly (p>0,05) deteriorated to the alphamesosabrobity (SI 2.54 2.62). On the contrary, in the inlet to the Gebhartsteich pond, where the value SI matched the alphamesosabrobity (SI 2.57), water quality in the outlet was insignificantly (p>0,05) slightly improved (SI 2.54), but the degree of saprobity has not changed. Similarly, in the inlet to the Pančár pond, where the SI value matched betamesosabrobity (SI 2.30), the outlet water quality was insignificantly (p>0,05) slightly deteriorated (SI 2.33), however again the degree of saprobity remained unchanged. Water quality changes after the flow through the investigated ponds resulted in the increase of the diversity index (H´) and vice versa (the worse water quality the lower H´). According to the analysis of the data of environmental conditions in organic and conventional carp ponds, they did not differ significantly. Also the species richness and the total number of individuals were not significantly different. The results of this diploma thesis demonstrated that the applied organic technologies of fish culture in monitored ponds did not have any significant impact (improvement) on the quality of aquatic environment and the composition of macrozoobenthos in the recipients of farm outflows, and also that no significant differences (deterioration) can be documented in the outflows from conventional ponds compared to the organic pond farming management.
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The food of pikeperch (Sander lucioperca) fry in ponds with different kinds of management
URBÁNEK, Marek
The aim of this study was a comphensive assessment of the food of pikeperch fry in the monoculture rearing from the stage of summer fry to the stage of autumn fry in ponds with three different types of management and assessment of the influence of individual treatments for pikeperch production. The experiment was performed in small ponds in experimental facility of FFPW in Vodňany. The variants of pond treatment were: a) ponds with additional prey fish (fry of common carp (C. carpio) and grass carp (C. idella)), b) ponds with installed substrate from heather, c) control variant without any support of natural food of pikeperch. All treatments had four repetitions. Rearing of pikeperch fry lasted 97 days. In all treatments, the opportunistic food strategy of pikeperch fry was recorded during the whole period of rearing and the main recorded food was macrozoobenthos, especially insect larvae. Bigger species of zooplankton had also been often found in the stomachs of pikeperch. Considerable development of macrozoobenthos in all experimental ponds was probably caused by the presence of submerged macrophytes. Thus, this factor made harder to estimate influence of installed substrates from heather for production of pikeperch fry and composition of its food. In the treatment with prey fish, the total length (F (9. 450) = 91.1; p < 10-3) and weight (F (9. 450) = 61.9; p < 10-3) of pikeperch fry were for the whole period of rearing significantly higher in comparison to the other treatments. In addition, the survival of pikeperch in the treatment with prey fish reached to 56 - 92,4 % at the end of rearing. In the treatment with substrate from heather, the total length and weight of pikeperch was significantly higher in comparison to the control treatment only in the first term of sampling. In the rest of pikeperch fry rearing period were the values comparable or higher in control treatment. Thus, for the rearing of pikeperch from the stage of summer fry to the stage of autumn fry is appropriate to stock prey fish in ponds. In comparison to installation of artificial substrate, the diversity of water environment, which is subsequently able to provide sufficiently wide natural food for reared pikeperch, seems to be more decisive.
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Effect of ponds and pond systems on the composition of the benthos in horní Lužnice
SVAČINA, Petr
The work is focused on monitoring changes in benthic communities in the longitudinal gradient of the river Lužnice. The river flows through to the pond area Třeboňsko and the largest pond of the Czech Republic - Rožmberk. It is examine affect of pond on benthic communities. The work involves collecting benthos according to the PERLA method on four profile in four seasons, the analysis of chemical parameters and data processing. The values of SI (saprobiological index) in the longitudinal gradient were from SI 1.8 to SI 2.6. SI values were at the outflow of Rožmberk higher than at inflow. The lowest values were in the first profile Suchdol (SI 1,8 ? SI 2,1) and higher values were in the last profile Vlkov (SI 2,4 ? 2,6). The chemical parameters at outflow were observed increased values of BOD5, TOC, NL105, NL505, chlorophyll and total P but lower values of nitrogen forms (Ntotal,NH4, N-NO3-). On the each profile was the significant change in the incidence or absence of certain groups. Profiles Suchdol and Hlína were diferent than Lužnice and Vlkov especially in the presence of stoneflies, dragonflies, beetles, and individuals generally indicates good water quality. Profiles at outflows from Rožmberk (Lužnice and Vlkov) showed a continued presence of bloodsuckers and more species indicating high organic pollution. NMDS method confirmed impact of sites in communities rather than period (R2 = 0.6 at significance level of p = 0.001).
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Effect of electrofishing on macrozoobenthos
PAVLÍČEK, Michal
Experiment has been addicted for observing effect of electrofishing on macrozoobenthos including mechanical stimulus (passing of trawling squad). Reaction on electrofishing and fading has been rebound by drift increment. Sample taking had been done by use of Surber and drift nets. Evaluation has been made both quantitative (standing crop in drift in particular methods), and qualitative (substitution of taxons in particular methods).
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The application of artificial substrates for quantitative and qualitative study of macrozoobenthos.
MAČEJ, Jakub
Macrozoobenthos are aquatic invertebrates, usually bigger than approximately 2 mm, living on the bottom. The larvae of aquatic insects (mayflies, midges, caddisflies, etc.), worms, molluscs and crustaceans belong among its most important representatives. The study was performed on a pond channel of the experimental pond system of the FFPW Vodnany. The artificial substrates, made of plastic blocks from biological filters, were installed in five rows of five blocks (replications) to monitor the process of their colonization in order to evaluate their suitability for the monitoring of macrozoobenthos. At weekly intervals, one series of five artificial substrates was sampled to collect colonizing macroinvertebrates for further elaboration (determination, numbers). It was confirmed that under the conditions of a pond canal, the colonization of artificial substrates by benthic animals begins immediately during the first week of their deployment. Species diversity of macrozoobenthos, expressed in number of taxa in successive weeks afterwards was not significantly different. The results proved that artificial substrates are suitable for quantitative and qualitative monitoring of benthic macroinvertebrate assemblage.
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Macrozoobenthos of the Pohořský and Dobechovský brooks in the Novohradské hory Mts.
KLOUDOVÁ, Jana
The presented thesis deals with the structure and composition of macrozoobenthos of Pohořský and Dobechovský brooks in the Novohradské Mountains. The evaluation was based on the collections from 10 localities carried out in July 2001, March 2002 and July 2002/2003. In addition to determination and classification of the species found, the collections have also been qualitatively evaluated with the use of the diversity index (saprobic index, Belgian Biotic index) and the similarity index. It has been found that the zoobenthos population of Pohořský and Dobechovský brooks encompasses the species of both trout and grayling zones. In most taxa it concerns characteristic representatives of clear, flowing and adequately aerated water. Mutual species similarity of macrozoobenthos population of Pohořský and Dobechovský brooks is approximately 40%. The comparison of macrozoobenthos population diversity indices of Pohořský and Dobechovský brooks has shown that the Pohořský brook bentic fauna is, according to the obtained data, richer and better balanced. From the saprobity point of view, the Pohořský brook can mainly be placed into oligosaprobic stream class. The water of Dobechovský brook oscillates between oligosaprobic and {$\beta$}-mesosaprobic level.
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