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National Repository of Grey Literature 2 records found  Search took 0.02 seconds. 
Vertical gradient of Hymenoscyphus fraxineus spore dispersal at infected locality in Boršov nad Vltavou
 ŠTOIDL, Petr
Hymenoscyphus fraxineus is a pathogenic sac fungus causing ash tree necrosis throughout Europe, spreading predominantly through the air using ascospores. The aim of this thesis was to quantitatively describe the occurrence of spores of the causative agent of ash trees necrosis at different heights above the source of infection. The results were subsequently used to consider the inoculum potential of this pathogenic fungus for longer distance spreading using air currents. The qPCR method was used for the detection and quantification of DNA concentration. Based on a statistical analysis of the obtained molecular data a dependence of the occurrence of different amounts of H. fraxineus on height was proved. The main contribution of this thesis lies in charting the range of the H. fraxineus pathogen spread in the air column which is directly related to the range of spores to greater distances.
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Detection of air-borne inoculum of Caliciopsis pinea in a plantation of Pinus radiata in Tuscany, Italy
 Bačová, Aneta
The genus Caliciopsis contains several representatives of the ascomycetous fungi which produce cankers on conifers. They comprise an interesting group with respect to morphology, physiology, and host response but have received relatively little attention in the past. The biology of this fungus is not totally elucidated. This study is focused on the description of seasonal spore dispersal of Caliciopsis pinea Peck and its relation to meteorological conditions which is needed for more precise and effective control of the disease. For this experiment the plantation of Pinus radiata in Carcheri (Lastra a Signa, Florence, Italy) was chosen. This plantation had symptoms of infection. A rotating arm spore trap and a weather station were installed here to obtain data from May to November 2016. Air samples were evaluated by qPCR with a very low detection limit. It has been found that spore traps are suitable for capturing the spore of this pathogen even with a very low incidence of inoculum in the air. Results show the presence of C. pinea inoculum throughout the entire sampling period with the highest peak in October. There is also a significant influence of precipitation on spore production. This work can help to better understand the life cycle of this pathogen. It can also contribute to enhance the control of this fungus and the protection of hosts.
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