|
|
Možnosti přežívání a množení patogenních a nepatogenních mikroorganismů v cukrářských výrobcích
Šrubařová, Monika
This master thesis is focusing on analysis of two kinds of confectionary products: nutty and lemon balls. The samples were analysed and they determinate the numbers of total count of bacteria, number of S. aureus, yeast and microscopic fungi, coliforms bacteria and E. coli, detection of Salmonella spp. and Listeria monocytogenes. Furthermore, S. aureus (strain CCM 5974) was inoculated in the samples. These samples determinate the number of S. aureus and possibility of the production an enterotoxins of S. aureus. Staphylococcal enterotoxin was detected with the reverse passive latex agglutination (RPLA). The result of determination is that the numbers of total count of bacteria influence of storage period the number was reduced (p<0,05). Statistical differences were not found between the nutty and the lemon balls (p>0,05). During the first and the second analysis was found growth of yeast and microscopic fungi in the nutty balls. In the lemon balls was found a growth of yeast and microscopically fungi during the first analyses and in one sample only. Also the number of bacteria was reduced with S. aureus (p<0,05). There were not found any statistical differences between the nutty and the lemon balls (p>0,05). The number of E. coli was not found in any our samples. The coliform bacterium was found only with the first and the second analysis in the nutty samples. In the lemon samples the coliform bacterium was found only in the first analysis. During the first detection of Salmonella spp. were found four colonies of this bacterium in the nutty samples. Colonies of Listeria monocytogenes were not detected in any of these samples. Microbiological analysis of inoculated sample confirms that our confectionary products are not suitable for growth of pathogenic microorganism S. aureus. It also proves that between the nutty and the lemon samples were no statistical difference (p>0,05). During the inoculation was proven that S. aureus (CCM 5974) with concentration 1,2.105 CFU.g-1 is capable to form in products enterotoxins, which stays in products in contrast with S. aureus, after a certain period of time.
|
|
|
Effect of packaging on the shelf life of meat products.
BURIANOVÁ, Michaela
The aim of this work is to compare the effect of different types of packaging (stretch film, modified atmosphere and vacuum packaging) meat products on their safety in terms of the standards that define specific requirements on health safety of meat products. The first part describes the development and various types of casings and packaging of meat products. The second part describes the implementation of the self-testing and measuring the occurrence of the microorganisms and coliform bacteria from their choice of suitable types soft meat products, types of packaging, sample preparation to evaluate the measured values. The results showed that the type of packaging is not the only criterion that affects the durability of selected meat products.
|
|
|
Testování antimikrobiální aktivity obalových materiálů s rostlinnými extrakty proti E. coli a koliformním bakteriím
Šabršula, Jan
This dissertation deals with evaluation and testing of food packiging materials antimicrobial aktivity that contain plant extracts and help in the fight against Eschericia coli and other coliform bacteria. This dissertation is divided into several parts. The first half of the work contains a literature review publishing about packaging materials containing plant extracts. It is also focused on literature review on the coliform bacteria, especially Escherichia coli genus, describes these microorganisms and their metabolic products, the effects of metabolic products which may negatively affect human health. Another part deals with measures to prevent undesirable microorganisms. The second half of the work has been engaged in actual testing of packaging materials containing plant extracts on relevant model organisms or directly on food. Data, acquired by testing are processed, compared with the figures in the available literatures and are listed at the end of work.
|
|
|
Analýza kvality pitné vody v jižních Čechách
VRÁNEK, Ivo
The goal of diploma thesis was a comparison of potable water indicators in representative areas of the south Bohemia. Monitored period had been from January 2012 till December 2014. Monitored parameters were microbiological indicators (Escherichia coli, coliform microbes, number of colonies at 22°C, number of colonies at 36°C, organoleptic indicators (color, turbidity, smell, taste). Physical and chemical indicators (ammonium ions, nitrates, nitrites, chemical consumption of oxygen, chlorides, conductivity, manganese, pH, sulphates, calcium and magnesium, iron). From the total complex of 1165 data, there were 23 values over the limit (1,97%) of evaluation of all monitored quality indicators (microbiological, organoleptic, physical and chemical). In evaluation of microbiological indicators, there were 6 values (1,97%) over the limit, from total 304 data. In organoleptic indicators, there was 1 value (0,44%) over the limit, from total 227 data. In evaluation of physical and chemical indicators, there were 16 values (2,52%) over the limit, from total 634 data. In total, there are 23 values which don´t meet determined limits from The Regulation No. 252/2004 and EU directives. There are these values of limits - number of colonies at 22°C, number of colonies at 36°C, turbidity, manganese, calcium and iron.
|
|
|
The analysis of selected parameters of water quality
KŘÍŽOVÁ, Iveta
Cílem této diplomové práce je provést analýzu vybraných ukazatelů kvality pitné vody a posouzení faktorů, které ji ovlivňují. V první části jsou zachyceny vybrané mikrobiologické ukazatele, tak jak je popisuje literatura, včetně platných norem a vyhlášek. Druhá část popisuje průběh provádění vlastní analýzy, z poskytnutých materiálů akreditovanou laboratoří společnosti AGRO-LA, spol. s r. o., až po vyhodnocení získaných výsledků.
|
|
| |
|
| |
guest ::
