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Microbiological diagnosis of Ureaplasma urealyticum and Mycoplasma hominis in women with urogenital infections
SLÍPKOVÁ, Gabriela
This bachelor's thesis is focused on the diagnosis of bacteria Ureaplasma urealyticum and Mycoplasma hominis in women with urogenital infections. The theoretical part of the work deals with urogenital infections, such as urinary tract infections, reproductive tract infections system of women and their most common bacterial agents. The issue is described in the next section Ureaplasma urealyticum and Mycoplasma hominis. The methodology and goals of the thesis included laboratory evidence of mycoplasmas, as well as evidence of sensitivity to antibiotics by using some kits. Another task was laboratory processing of swabs from the vagina. Furthermore, the statistical evaluation of the results of patients in Unilabs k.s. laboratory. Statistical evaluation led to the detection of common presence of Ureaplasmas and Mycoplasmas with some other microorganisms inhabiting the fiale urogenital tract. For the statistical part, I used the results of the patients for the period 1/1/2019 - 12/31/2022. I had a total of 859 results available. Based on them, I found out that the largest number of positive findings predominates Ureaplasma urealyticum, followed by a mixed infection of Ureaplasma urealyticum and Mycoplasma hominis to a lesser extent. Only in small number have been found positive Mycoplasma hominis. Among the associated microorganisms, the most frequent occurrence of lactobacilli was associated with Ureaplasma urealyticum positivity. Gardnerella vaginalis was found in all positivities with mycoplasmas, the highest in Ureaplasma urealyticum. Furthermore, Streptococcus anginosus and Candida albicans are represented to a similar extent, again to the greatest extent represented in the case of Ureaplasma urealyticum positivity. Other microorganisms were represented to a lesser extent and are described in more detail in the results of the thesis.
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Leucocytozoon in passerines
Geržová, Lenka ; Svobodová, Milena (advisor) ; Vinkler, Michal (referee)
Leucocytozoon is the least studied genus of blood parasites of the order Haemosporida comparison with the genera Plasmodium and Haemoproteus. Its transmission is almost exclusively linked with a vector of the genus Simulium, which ensures the circulation of parasites in populations of wild and domestic birds. Numerous species have been are described by classical taxonomy, but it is assumed that this genus hides a number of cryptic species, its diversity being much higer. Methods of detection include microscopy, a classical method used for many decades, and molecular biology methods introducted at the turn of the millenium. Key words: Leucocytozoon, passerines, prevalence, species, dynamics of infection, detection
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Influence of environment on distribution and coexistence of different diseases on a single plant species
Mráčková, Michaela ; Koubek, Tomáš (advisor) ; Koupilová, Klára (referee)
Most plant species host one or multiple pathogens. However, studies are mostly focused on simple pathosystems. These studies deal for example with the virulence of the pathogen, plant resistance or influence of the environment on the pathosystem. The occurrence of more pathogens in one plant species is taken into account only in a few studies. Most of those studies focus on the coinfection of multiple pathogens in one individual host, especially in the agriculture, so there is very little information about coexistence of more pathogens in wild plant population. This paper summarizes the general information about plant and pathogen interaction, focuses on environmental effects and finally focuses on occurrence of multiple pathogens on one plant species. From the studies it is clear, that both, abiotic (light, temperature, humidity, etc.) and biotic conditions (pollinators or pests as vectors etc.) have impact on host- pathogen interaction. During co-occurrence of multiple pathogens in one host plant, various interactions between coinfecting pathogens can occur (competition, cooperation, etc.). Resistance of plant to one pathogen can be affected by presence of other pathogens and stress factors. Finally, coinfection may also affect virulence of individual pathogens. Key words: plants, pathogens,...
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