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Barcoding společenstva makrozoobentosu Křesánovského potoka
PEJCHAROVÁ, Eliška
Molecular genetic methods, including barcoding, have been increasingly utilized in recent years for the analysis of communities of macrozoobenthos and many other organisms. Morphological identification of precise species of mayflies, caddisflies, or stoneflies in their larval stage is demanding and requires a significant amount of time and expertise. Barcoding analysis can help to classify disputed species more accurately. The aim of this thesis is to use the barcoding method to determine selected representatives of macrozoobenthos and verify the morphological determination of macrozoobenthos representatives. The studied locality is Křesánovský Stream in southern Bohemia. DNA was extracted from the collected samples and using mitochondrial gene CO1 and primer pairs LCO1490, HCO2198, jgLOC1490, and jgHCO2198, sequences were created and tested in the BLAST program. The most similar sequences from publicly available databases were obtained, compared to sequences from this thesis and the exact species were subsequently determined. A total of 57 samples were studied, from which 29 species were morphologically identified (six species of mayflies, eight species of caddisflies, seven species of stoneflies, three representatives of beetles, four species of dipterans, and one specimen of Gammarus fossarum), and 30 species were identified through barcoding (11 species of mayflies, eight species of caddisflies, five species of stoneflies, two species of dipterans, three species of beetles, and one species of fleas). Morphological determination was successful in 63% of cases. The success rate was 74% for mayflies, 67% for caddisflies, 33% for stoneflies, and 25% for dipterans. The specimen of Gammarus fossarum was morphologically identified correctly. Barcoding appears to be a suitable method for studying macrozoobenthos and an excellent complement to morphological analysis of samples. However, it has its limitations, and it is not appropriate to consider it as the sole method for determining macrozoobenthos samples.
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Diverzita a speciace jepic (Ephemeroptera) z Kavkazu a přilehlých oblastí
HRIVNIAK, Ľuboš
This dissertation thesis is focused on the diversity and evolutionary history of mayflies (Ephemeroptera) from the Caucasus, one of the most biologically diverse areas of the Palaearctic realm. The introduction details general information about the evolutionary mechanisms leading to the high diversity of mountain biota, the processes driving evolutionary diversification in aquatic insects, and the current state of knowledge on the diversity and distribution of mayflies in the Caucasus. The core of the thesis consists of five published articles and one manuscript, which are divided into four chapters. The first chapter deals with the diversity and distribution of mayflies from Armenia, the second and third are focused on diversity, integrative taxonomy, and identification of Epeorus (Caucasiron), one of the most diverse mayflies in the Caucasus. The last chapter is focused on the origin, historical biogeography, and evolutionary diversification of Caucasiron, the model group selected for the evolutionary study of mayflies from the Caucasus. The chapter describes when and under which circumstances Caucasian mayfly endemism and diversity developed. The thesis is concluded with a detailed summary of the results, and future perspectives in the research of Caucasian mayflies.
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Variability of Ephemeroptera of family Baetidae (Insecta, Ephemeroptera, Baetidae)
KOLLROSS, Jan
This thesis deals with a variability of the Baetis fuscatus species-group (Ephemeroptera: Baetidae). This study contains three main topics: The first one is distinguishing between two highly similar species, Baetis scambus and Baetis fuscatus based on morfological structures, specifically their mouthparts - labial palps. To analyze the shape of the labial palps, I applied a computerized outline analysis. Second topic is focused on the validity of Baetis beskidensis as a distinct species based on the morphology and DNA sequences (obtained for the topotypical material for the first time). And finally, I also investigated some samples from Corsica in attempt to discover new species of the Baetis fuscatus species-group. I found that most probably it is not possible to distinguish between Baetis scambus and Baetis fuscatus based on the shape of their labial palps. Baetis beskidensis is morfologically different, but genetically not distinctly separated from other representatives of the Baetis fuscatus species-group. I didn´t discover any distinct morphological differences in specimens from Corsica, pointing to the possible existence of new species.
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Systematics of mayflies (Ephemeroptera) of the family Baetidae
SROKA, Pavel
The current thesis reviews a cross-section of studies dealing with several problems of systematics of the mayfly (Ephemeroptera) family Baetidae. It is based on classic morphological characters as well as molecular-based methods in order to solve specific taxonomic problems and reconstruct phylogenetic relationships within the selected taxa.
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Species variability of bentic organisms in Dračice river classicifed by PERLA system
KOUTNIK, Dalibor
Examination ecological size selected part of river (from village Františkov to border with Austria) on the basis of field watching. Observed results are analysed by system PERLA. Method is based on analysing biodiversity makrozoobenthos sample from fordables running water. Sampling can be apply in running water, where water column not exceed 1 m and speed of water stream to 1 m/s. Taking is made by normal or low water stage.
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