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Expression and localization of alpha V and alpha 3 integrins in male germ cells of selected mammalian species
Bajerová, Kateřina ; Frolíková, Michaela (advisor) ; Krejčová, Tereza (referee)
Integrins are transmembrane glycoproteins mediating cell-cell adhesion and ensuring communication between extracellular matrix and cell cytoskeleton. The presence of integrin subunits has already been described on somatic cells, as well as on oocytes and sperm cells. In oocyte plasma membrane, integrins together with tetraspanins are involved in the formation of vast molecular webs. Analogical structures have been recently discovered in sperm plasma membrane as well. While the role of integrins on oocyte has been researched quite extensively, there is much less known about its presence and function on sperm. This Master's thesis focuses on expression and localization of integrins alpha V and alpha 3 on acrosome-intact sperm and its comparison between mouse, pig and human sperm cells. Furthermore, this thesis also describes relocation of the alpha V integrin after the acrosome reaction, which is an essential step of sperm maturation. Using confocal microscopy, the alpha V integrin localization was detected in acrosomal cap area of acrosome-intact sperm of all selected species. After acrosome reaction, the alpha V integrin relocated and was depicted in postacrosomal region and equatorial segment of acrosome-reacted sperm. Immunodetection revealed a decreasing expression of the alpha V integrin in...
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