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Význam signální dráhy insulinu ve fyziologii klíštěte \kur{Ixodes ricinus}
KOZELKOVÁ, Tereza
In this thesis, the function of the insulin receptor signaling pathway (ISP) in hard ticks Ixodes ricinus was analyzed. Ticks are obligatory blood-feeding (hematophagous) ectoparasites, capable of transmitting a wide variety of pathogens comprising bacteria, viruses, and protozoa that affect animals and humans. The parasite is strictly bonded with its host through a unidirectional transfer of nutrition for its survival, development, and reproduction. The ISP is a highly conserved system, which regulates a variety of physiological and anabolic processes in response to the available nutrition. The aim of the thesis was to examine the function of several key components of this pathway, which had been identified in the midgut transcriptome, namely insulin receptor (IrInR), protein kinase B called AKT (IrAKT), and the target of rapamycin (IrTOR). The subsequent objective was to assess the expression profiles in tick tissues of these components, during tick feeding and after detachment using qRT-PCR. Furthermore, the phenotype using RNAi knockdown, injection with insulin receptor antagonist (IRA), and the artificial feeding with the AKT and TOR inhibitors were verified. Finally, the immunization of rabbits with IrInR recombinant protein and the tick infestation were carried out.
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