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Adaptability of electrospun nanofibers for bone-tissue engineering
Havlíková, Tereza ; Kaspar, Pavel (referee) ; Papež, Nikola (advisor)
The field of bone tissue engineering focuses on developing biocompatible scaffolds, which can successfully mimic the extracellular matrix of human tissue and provide the growing bone cells with the necessary mechanical support and adequate stimulation for proper growth and proliferation. This bachelor thesis describes the basic principles of tissue engineering with an emphasis on the area of bone tissue regeneration, examines current technological challenges and discusses prospective materials and technologies for the design of a compatible tissue scaffold. Electrospun nanofibers, their characteristic properties and adaptability are discussed in more detail. Based on the theoretical findings from the research, a design of a suitable scaffold created from the selected material for following seeding of bone cells, and the planned production procedures are presented. According to the design, the tissue scaffold is manufactured and a series of fundamental research analyses are conducted on it in terms of its structural integrity, chemical composition, wettability, crystalline phase content and cell adhesion. The results of these analyses inform the conclusion regarding the tissue scaffold’s adaptability for bone tissue engineering.
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Adhesion, growth and differentiation of osteoblast-like cells on materials for bone implants
Doubková, Martina ; Bačáková, Lucie (advisor) ; Filová, Eva (referee)
This thesis focuses on testing and improving Ti-6Al-4V ELI biomaterials, which are currently one of the most used titanium alloys in biomedicine (predominantly in orthopaedics and dentistry), in cooperation with research institutions and private companies developing and producing such materials. The metallic samples were previously modified by plasma electrolytic oxidation (PEO) with use of electrolytes of a different composition to induce development of a homogeneous TiO2 layer on its surface. In vitro interactions of human osteoblast-like cell line Saos-2 with the surface of Ti-6Al-4V ELI alloy samples are investigated. Initial cell attachment, spreading, morphology, cell population density, viability, calcium deposition and expression of selected osteogenic markers, e.g. collagen type I, alkaline phosphatase and osteocalcin, were evaluated on cultured cells. The cells behavior were then correlated with physicochemical properties of the material surface, such as its topography, roughness, wettability, surface layer chemical composition etc. The results are also compared with those obtained in cells cultured on control samples of untreated alloys as well as microscopic glass coverslips and bottom of standard polystyrene cell culture wells. The aim of this thesis is to select the most promising...
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