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Excited state dynamics of carotenoids in solution and proteins
CHÁBERA, Pavel
Time resolved spectroscopy is one of the crucial methods used to study processes on molecular level in biological systems. It is useful especially for monitoring fast processes that take a place in photosynthetic apparatus of photosynthetic organisms, such as electron and energy transfer. The integral parts of photosynthetic apparatus are carotenoids, whose role in the photosynthetic apparatus is not as well explored as it is for chlorophylls. It was proved that carotenoids actively participate in energy transfer processes in photosynthetic antennas. They have a crucial role in protection against excess energy damage. They are also electron donors in both antennas and reaction centers. The fact that photo-physical properties of carotenoids are much different from properties of others organic pigments, complicates studies of their functions in photosynthesis as well as in other biological systems. This thesis employs advanced methods of femtosecond spectroscopy to obtain more information about carotenoid functions in some biological systems and in solution with special focus on carotenoids containing carbonyl group.

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