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Comparing results of cell blood count and leukocyte differential count between different series of analyzers Sysmex
Opletalová, Monika ; Blažková, Hana (advisor) ; Bártů, Iva (referee)
In this bachelor thesis I focused on comparing results of cell blood count and leukocyte differential count between three types of Sysmex analyzers. XS - 1000 i, XT - 4000i and XE - 5000 are automatic analyzers, measuring on principles of flow cytometry and impedance methods using hydrodynamic focusation. These methods provide very accurate blood cell an alysis. The theoretical part of bachelor thesis is focused on description of hematopoesis, options for measuring cell blood counts and leukocyte differential counts and general description of measuring methods used by Sysmex analysers. In the experimental part I described the composition of mentioned analysers, their measuring channels and reagents which are used for peripheral blood cells analysis. Every sample from 50 patients was measured on each analyser and results were statistically compared by using ANOVA test. Graphs showing average values of each parameter for negative and positive patient ́s samples were made. The aim of this study was to summarize the main advantages and disadvantages of all three analyzers and determine whether differences in the results of samples between analyzers are statistically significant or not. Powered by TCPDF (www.tcpdf.org)
Comparing results of cell blood count and leukocyte differential count between different series of analyzers Sysmex
Opletalová, Monika ; Blažková, Hana (advisor) ; Bártů, Iva (referee)
In this bachelor thesis I focused on comparing results of cell blood count and leukocyte differential count between three types of Sysmex analyzers. XS - 1000 i, XT - 4000i and XE - 5000 are automatic analyzers, measuring on principles of flow cytometry and impedance methods using hydrodynamic focusation. These methods provide very accurate blood cell an alysis. The theoretical part of bachelor thesis is focused on description of hematopoesis, options for measuring cell blood counts and leukocyte differential counts and general description of measuring methods used by Sysmex analysers. In the experimental part I described the composition of mentioned analysers, their measuring channels and reagents which are used for peripheral blood cells analysis. Every sample from 50 patients was measured on each analyser and results were statistically compared by using ANOVA test. Graphs showing average values of each parameter for negative and positive patient ́s samples were made. The aim of this study was to summarize the main advantages and disadvantages of all three analyzers and determine whether differences in the results of samples between analyzers are statistically significant or not. Powered by TCPDF (www.tcpdf.org)

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