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Monitoring of Paenibacillus larvae, Melissococcus plutonius and Ascosphaera apis pathogens in honeybee colonies in the Czech Republic
JOSKOVÁ, Markéta
The aim of this thesis was to summarize the theoretical informations about biology of honeybees and bacteries Paenibacillus larvae and Melissococcus plutonius and parasitic fungi Ascosphaera apis. This organisms attack bee larvae and cause bee diseases. The major part of this thesis was focused on the evaluation of the occurrence of these pathogens in various places in Czech Republic by isolating bee DNA from more than 200 bee samples from 50 locations within whole Czech Republic. Detection pathogens was performed using PCR method. There were 3 samples positive for Paenibacillus larvae and 4 positive for Melissococcus plutonius. The fungus Ascospheara apis did not occur in any of the samples.

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