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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Využití vysokorozlišovací cytometrie ke studiu živých buněk
 Vařecha, M. ; Amrichová, J. ; Ondřej, Vladan ; Lukášová, Emilie ; Kozubek, Stanislav ; Matula, Pa. ; Matula, Pe. ; Kozubek, Michal
The fluorescent proteins are important innovation in the field of cell biology and in vivo experiments that use fluorescent proteins bring new interesting results from a different point of view than in vitro and in situ experiments. This presentation will introduce our automated 2D/3D high-resolution time-lapse image acquisition and analysis of living cells transfected with plasmids encoding fusion proteins or dyed with fluorescence probes. One of our in vivo projects, we will mention, is focused on the study of mitochondrial and nuclear apoptotic processes in living cells. Another in vivo project concentrates on the study of topography of telomeres and incidence of telomere-association phenomenon in various types of human tumor and healthy cells. In our experiments, cells are stably or transiently transfected with plasmid DNAs coding mitochondrial apoptogenic or telomere-binding proteins with fluorescent proteins.
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Rychlé snímání obrazu živých buněk a jeho limity
 Matula, Pa. ; Kozubek, Michal ; Kozubek, Stanislav ; Ondřej, Vladan
Technique of high-resolution cytometry (HRCM) developed in our laboratory is capable of automated (2D as well as 3D) acquisition and analysis of fluorescent stained cells. The cytometer can process large number of cells with high resolution. The acquisition can be repeated in time and that enables live cell studies. If very short events in living cells are studied then the sampling frequency must be high. The highest sampling frequency depends on the technical parameters of the motorized parts of cytometer (especially on the camera frame rate, the speed of filter exchange and the speed of axial movement) and on the control software, which must be appropriately optimized. The limits of the current setup are discussed and available sampling frequencies for different acquisition modes are listed.
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