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Comparison of haematological parameters selected groups of reptiles
Jenčík, Filip ; Vrabec, Vladimír (advisor) ; Koleška, Daniel (referee)
The thesis focuses on the comparison of selected groups of reptiles with respect to the composition of blood. The first part of the thesis analyses selected available reference works from the current perspective of understanding in both domestic and international sources. A great deal of attention is paid to the procedure of collection of blood samples, preparation of blood cell films and their analysis.
The thesis also comprises voluminous information on qualitative and quantitative aspects of haematology of lizards, turtles and snakes. The issue of differences in haemogram of selected group of reptiles is tackled taking into consideration the facts influencing the haemogram of the reptiles, such as the age and size of reptiles, seasonal changes, hibernation, the impact of the environment, nutrition as well as the method of breeding in - captivity or free range.
The second part of the work concentrates on the collection of blood samples, preparation of blood cell films, identification of blood elements of green iguana Iguana iguana (Linnaeus, 1758), red-eared slider Trachemys scripta (Thunberg et Schoepff, 1792) and red-tailed boa Boa constrictor (Linnaeus, 1758). The samples collected from these creatures were analysed as for representation of blood components (erythrocytes, haematocrit, haemoglobin, leucocytes, heterophils, lymphocytes, monocytes, azurocytes, eosinophils, basophiles) and the measured values were compared against the published irreferential values and within the group of these creatures.
Having analysed the blood cell films we have arrived at the conclusion that the morphological features of blood cells of the examined reptiles were identical with the parameters set out in the literature. Morphology of the blood cells of the monitored reptiles varies. Our results indicate that blood count as well as differential blood count of the examined creatures fits in the limits of the published referential works. The examined samples confirm the differences in numbers of individual types of blood cells of lizards, turtles and snakes. In summary it would be desirable to examine a larger number of creatures in order to obtain the optimal number of results.
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