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Methods of Bloodcells Counting
Pavlík, Jan ; Provazník, Ivo (referee) ; Hrubeš, Jan (advisor)
At present, the standardization of morphological examination of cytological preparations appears to be very difficult. Digitization and development of computer technology makes it possible to find a suitable solution for the effective processing of cytological samples. Company Cellavision, in collaboration with leading morphologist, developed a device Cellavision DM96, which uses image capture of blood cells for their morphological diagnosis and subsequent archiving. It is of great importance to focus on this system and examine its use in clinical practice. The results obtained by two different methods were statistically processed and evaluated.
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Experimental infection of Oryctolagus cuniculus with fluke Fascioloides magna
Melounová, Klára ; Kašný, Martin (advisor) ; Novobilský, Adam (referee)
Fasioloides magna is a trematode parasitizing in the liver parenchyma of ruminants. Its life cycle is associated with the humid environment and includes intermediate freshwater snail hosts from family Lymnaeidae. According to the ability of host to form a certain type of a pseudocyst during fascioloidosis, they can be,divided in three groups, specific definitive hosts (red deers, fallow deers, roe deers), nonspecific definitive hosts (cattle, wild boars and elks) and atypical hosts (sheeps and goats). Beside the natural infections also the experimental infections of other potential host species has been realized (chamois, llama and bighorn sheep and traditional laboratory animals such as mice, guinea pigs, rats and rabbits). In the context of different diseases, many changes in infected organism can occur. These can be qualitatively and quantitatively evaluated. Similarly, during fascioloidosis the changes associated with the presence of the parasite in the host's body is possible to monitor, e.g. antibody production, increase in the number of eosinophils, release of eggs in faeces, internal bleeding, or the level liver damage. The liver damage is corresponding primarily to biochemical parameters of blood, not only the liver enzymes, but also other blood components, like blood proteins, lipids,...
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Application of Haematological Methods in Zoological Studies
Poplová, Jitka ; Vinkler, Michal (advisor) ; Krulová, Magdaléna (referee)
Haematological methods are widely used in zoological researches. Unfortunately, especially in ecological studies there is often much imperfection in techniques adopted, and misinterpretation of results is also common. This is particularly the case of methods serving to assessment of the peripheral blood cellular composition. In this thesis I therefore decided to describe basic haematological methods, their mechanisms, advantages and disadvantages. I also highlighted possible application potential in several less widely utilised haematological techniques. In the practical part of this thesis I have shown that the examination of immature erythrocyte differential count may serve as a meaningful indicator of health state in birds.
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The importance and methods of measuring hematocrit and hemoglobin in pre-hospital emergency care
PEŠL, Stanislav
This bachelor thesis is called: "The importance and methods of measuring hematocrit and hemoglobin in pre-hospital emergency care". There has been recent progress in technologies which allows to measure various laboratory values by small portable systems. These values can be Hb and Ht, measuring of these values is not standard in pre-hospital medical care in Czech Republic. It is the reason why this thesis deals with possibility of its implementation. The theoretical part deals with composition and physiology of hemoglobin and associated diseases such as anemia and polyglobulia. It also contains the issue of diagnostics, guidelines of physical examination procedures as well as description of what should be focus on when one is gathering case history. There are also provided facts and information about portable measuring systems, their measuring methods and overview of systems which are available in Czech Republic. There was conducted a qualitative research and was presented in practical part of this bachelor thesis. Collection of data was based on technique of standardized anonymous questionnaire which included only structured answers. The paramedics of Emergency medical services of South Bohemia region were the respondents. The research took place in March 2014 and 54 respondents anticipated in it. There were stated two aims in this bachelor thesis. The heart of first aim was to find out whether the paramedics are able to use values of hematocrit and hemoglobin to diagnose a patient. The second aim was to map paramedics' attitude to measuring values of hematocrit and hemoglobin within emergency medical service. There were also stated hypothesis which expressed the expectations that paramedic are able to use values of hematocrit and hemoglobin and they are interested in and enthusiastic about measuring hematocrit and hemoglobin within pre-hospital emergency care. From data, which were collected and analyzed in the practical part, it is obvious, that paramedics do have satisfactory amount of skills and knowledge which are absolutely necessary for diagnosing a patient by using values of hemoglobin and hematocrit. Contrary to the fact that paramedics have certain knowledge about benefits which would measuring values of hemoglobin and hematocrit brought they do not believe that portable measuring systems would be efficient in pre-hospital emergency care and they are not interested in using them.
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Methods of Bloodcells Counting
Pavlík, Jan ; Provazník, Ivo (referee) ; Hrubeš, Jan (advisor)
At present, the standardization of morphological examination of cytological preparations appears to be very difficult. Digitization and development of computer technology makes it possible to find a suitable solution for the effective processing of cytological samples. Company Cellavision, in collaboration with leading morphologist, developed a device Cellavision DM96, which uses image capture of blood cells for their morphological diagnosis and subsequent archiving. It is of great importance to focus on this system and examine its use in clinical practice. The results obtained by two different methods were statistically processed and evaluated.
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Seasonal dynamics of selected blood parametres of selected flesh breeds of sheep bred in foothills conditions
ŽÁČKOVÁ, Klára
Sheep breeding is nowadays a developing branch of agriculture again. There is a lot of different breed and they react distinctly on the same conditions of the enviroment. Sheep of breeds charollais, suffolk, šumavská ovce and valaška bred in similar conditions were observed in spring and autumn of years 2007 and 2008. In these seasons were taking blood samples (from {$\pm$}7{--}24) ewes and lambs and were analyzed in hematology laboratory. There were determined haemoglobin level, haematocrit indicator, erytrocytes and leucocytes levels, glucose, cholesterol, triglycerides levels, urea and plasmatic proteins, activity of ALP and GMT enzymes, phosphor, calcium, magnesium, zinc and copper levels. The main objective of this project was determine seasonal changes in observed parametres. Next objectives were determine different changes in blood parametres in different breeds and different aimes of breeds. There were recognized that all the observed breeds don`t react the same way on similar conditions. There were not provably determined seasonal changes in observed parametres, but average Hb level was higher in autumn than in spring. Urea level was conversely higher in spring season than in autumn. The demostrable fact is, that the similar conditions induce different answers not only in different breeds but also in different aimes of breeds.
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