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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Genetic potential for methane metabolism in the Greenland subglacial ecosystem
 Rybár, Marek ; Stibal, Marek (advisor) ; Šabacká, Marie (referee)
Subglacial environments, located at the interface of glacier ice and bedrock, represent one of the major ecosystems associated with glaciers and ice sheets. This environment contains liquid water and underlying sediment with large amounts of organic matter overridden during periods of ice advance. Large areas of subglacial sediment are exposed annually by glacier melting and retreat, which affects the subglacial carbon stores and may significantly impact regional carbon fluxes. Due to the widespread anoxia at glacier beds, subglacial environments are potential producers and reservoirs of methane that can be released into the atmosphere as a consequence of glacial retreat. While the presence of methanogens and methane oxidisers has been shown based on 16S rRNA gene data, no data on the functional genetic potential for methane metabolism currently exist. In this study, the first subglacial metagenomes obtained from subglacial sediment exported from beneath the Greenland ice sheet by a meltwater river were used to characterize the metabolic potential for methane metabolism by the identification and quantification of genes involved in methane production and consumption. Functional genes of potential syntrophic pathways were also investigated. Functional genes of methanogenesis/anaerobic oxidation of methane and...
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Microbial communities and carbon cycling in subglacial ecosystems and their analogues
 Rybár, Marek ; Stibal, Marek (advisor) ; Řeháková, Klára (referee)
Subglacial environments are located at the interface of glacier ice and bedrock. They represent one of the major ecosystems associated with glaciers and ice sheets. They contain liquid water and fine material including organic matter, accumulated during periods of glacier advance. It is well established that there are active microbial communities residing in these environments, which are adapted to living in extreme conditions. Subglacial ecosystems are mostly isolated from the atmosphere and thus their oxygen content is usually very low. Therefore, the organisms residing in these environments often employ anaerobic/anoxic strategies to ensure their survival. However, knowledge of these communities is limited due to practical constrains associated with subglacial ecology and biogeochemistry research. The activity of microorganisms beneath glaciers significantly influences carbon cycling. In recent years, this ecosystem is dynamically changing and may have important impact on regional and global carbon cycle. Therefore, it is important to better understand this ecosystem. This thesis summarizes recent knowledge of microbial communities and carbon cycling in this ecosystem and discusses suitable analogues, which could help us understand the fascinating subglacial ecosystem and formulate future research...
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