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Detection of disease caused strain Xanthomonas phytopathogenic for tomato and pepper by multiplex PCR
RAGOZINA, Mariia
The subject of this work is the optimization of multiplex PCR for the detection of pathogenic bacteria of the genus Xanthomonas. Were successfully managed to optimized multiplex PCR reaction for simultaneous detection of Xanthomonas gardneri, Xanthomonas perforans, Xanthomonas euvesicatoria, and Xanthomonas vesicatoria using four primer pairs: Bs-Xg (Koenraadt et al., 2007), Bs-Xp (Koenraadt et al., 2007), Xe_330CG1,2 (Stehlíková a Beran, unpublished) and Xv_367CG1-1,9 (Stehlíková a Beran, unpublished). The selected primer pairs were also tested on a wide scale of other bacteria of Xanthomonas genus and non-Xanthomonas pathogens for tomatoes and peppers.
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Design and testing of multiplex-PCR primers for detection of bacterial spot of tomato
STEHLÍKOVÁ, Dagmar
The subject of this work is to develop multiplex-PCR assay for specific detection of plant pathogenic bacteria of Xanthomonas genus causing bacterial spot of tomato. PCR primers for detection of groups A (X. euvesicatoria), B (X. vesicatoria), C (X. perforans) and D (X. gardneri) were developed based on the DNA sequences obtained by sequencing and from the GenBank database (NCBI). Four primer pairs - Xe_shotgun_104, Xe_shotgun_1819, Xv_atpD_403, Xp_efP_202 were designed and subsequently thoroughly tested and optimized for parallel detection of these bacteria. Specificity of the primers was tested on a large complex of bacterial strains pathogenic to tomato and related crops. Following the protocol described above X. vesicatoria, X. euvesicatoria, X. perforans and X. gardneri can be quickly and reliably identified in a single multiplex-PCR assay.
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