|
|
Study of the autophagosome biogenesis in Arabidopsis
Ničová, Veronika
The process of autophagy is a cellular mechanism that allows cells to dispose of both damaged and unwanted components. These components are covered by a double membrane, from which an autophagosome arises. Afterwards, the content is transported to the vacuole, where it is degraded or recycled. Due to the immobility of plants, they have evolved a number of mechanisms for defending themselves from adverse conditions. It is well known that autophagy occurs throughout the life of a plant, but it can be intensified if needed, for example in the event of a stressful condition. In view of the fact that autophagy is a relatively complex regulated mechanism, there is an ongoing effort to conduct research on this topic. Therefore, autophagy is important in stress condition responses, whether they are caused by biotic or abiotic factors. There are various types of stress that can influence autophagy in different ways, including poorly packaged proteins or the availability of nutrients. Additionally, autophagy is not only essential for the tolerance to certain applied stresses, but it is also essential for the regulation of stress memory. Functional characterization of phosphatidylinositol-3-phosphates role in autophagy and phagophore biogenesis is also very important. As part of this thesis, the mtv11 mutant plant was monitored. The mutant plants carry a mutation in the MTV11 gene that encodes an important subunit of the phosphoinositide 3-kinase complex. Plants were monitored under abiotic stress conditions to study their effects on autophagosome formation. A study of this mutant plant might be contributing to the accurate understanding of the autophagosome biogenesis.
|
guest ::
