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Fluorescent nanoparticles based on organic chromophores
Čekal, Josef ; Kratochvíl, Matouš (referee) ; Vala, Martin (advisor)
This study aims to create Host-Guest nanoparticle systems exhibiting white light emission (WLE) using organic molecules, achieved through Förster resonance energy transfer (FRET) mechanisms. Substituted stilbene-based compounds were utilized for the preparation of nanoparticle systems, with CA-DPS-V (H2) and CA-DPS-CHO (H1) used as donor/matrix materials, and CA-DPS-DCV (G1) and DPA-DXS-IOO (G2) as acceptor/dopant materials. Three nanoparticle systems were synthesized via nanoprecipitation from common solutions of the compounds in THF: system 1 (H1+G1), which did not achieve WLE, system 2 (H2+G1), and system 3 (H2+G2). Systems 2 and 3 were characterized using fluorescence spectroscopy and FRET parameter calculations. It was found that the Förster distance in system 2 is shorter than in system 3. This variation in Förster distance affects the composition ratios capable of WLE. The closest proximity to the white point (0.33, 0.33) was observed in system 2 at molar ratios of 110:1 (0.32, 0.38) and 164:1 (0.29, 0.32), and in system 3 at ratios of 340:1 (0.34, 0.30) and 476:1 (0.31, 0.28). Thus, the goal of the study was achieved, demonstrating that nanoparticles capable of WLE can be prepared in this manner. However, a drawback of the prepared nanoparticles was their low photostability. Future work could focus on synthesizing more stable nanoparticles from structurally similar compounds. These particles could then be further characterized in terms of structure and other properties for potential applications.
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Loudness Index Measuring
Hloušek, Pavel ; Mach, Václav (referee) ; Kurc, David (advisor)
This thesis deals with noise measuring. There are described methods of measurement and evaluation of noise, focusing on Steven’s loudness calculation method. Basic concepts of acoustics are provided in the first part. There are also mentions measurement of noise using microphones and sound level meter. The second part is focused on the practical solution of thesis. In which it is made measurement of noise using sound level meter NTi Acoustilizer and implementation of the graphical user interface for calculation of the Steven’s loudness index in Matlab.
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Surface-enhanced Raman microspectroscopy of biomolecules and biological systems
Šimáková, Petra ; Procházka, Marek (advisor) ; Michl, Martin (referee) ; Šloufová, Ivana (referee)
The aim of this thesis was using surface-enhanced Raman scattering (SERS) microspectroscopy for the study of biomolecules and biological systems. The main probe molecule was cationic porphyrin H2TMPyP, however, other porphyrins, tryptophan and two lipids were also used. The sensitivity and reproducibility of several solid SERS substrates: (i) Au and Ag nanoparticles (NPs) immobilized via a bifunctional linker, (ii) AgNPs immobilized by drying, (iii) highly ordered Au and Ag film-over-nanosphere (FON) and (iv) Ag-coated insect wings were compared. On most of the solid substrates, the lowest detected H2TMPyP concentration was ~10-8 M. The highest sensitivity was provided by the dried drops of AgNPs/analyte mixture, where concentrations 1×10-10 M TMPyP, 1×10-5 M tryptophan, 2×10-7 M DSPC and 3×10-7 M DMTAP were detected. Nevertheless, the spectral reproducibility was decreased due to porphyrin metallation and perturbation of the lipid spectra in comparison to their Raman spectra from solution. The highest reproducibility was achieved by AuFON and Ag-coated insect wings. Finally, the AgNPs modified by PEG polymers were tested for intracellular application using HeLa cancer cells. Metallation of H2TMPyP served to probe the accessibility of PEG- AgNPs surface. The results proved that the accessibility...
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Surface-enhanced Raman microspectroscopy of biomolecules and biological systems
Šimáková, Petra ; Procházka, Marek (advisor) ; Michl, Martin (referee) ; Šloufová, Ivana (referee)
The aim of this thesis was using surface-enhanced Raman scattering (SERS) microspectroscopy for the study of biomolecules and biological systems. The main probe molecule was cationic porphyrin H2TMPyP, however, other porphyrins, tryptophan and two lipids were also used. The sensitivity and reproducibility of several solid SERS substrates: (i) Au and Ag nanoparticles (NPs) immobilized via a bifunctional linker, (ii) AgNPs immobilized by drying, (iii) highly ordered Au and Ag film-over-nanosphere (FON) and (iv) Ag-coated insect wings were compared. On most of the solid substrates, the lowest detected H2TMPyP concentration was ~10-8 M. The highest sensitivity was provided by the dried drops of AgNPs/analyte mixture, where concentrations 1×10-10 M TMPyP, 1×10-5 M tryptophan, 2×10-7 M DSPC and 3×10-7 M DMTAP were detected. Nevertheless, the spectral reproducibility was decreased due to porphyrin metallation and perturbation of the lipid spectra in comparison to their Raman spectra from solution. The highest reproducibility was achieved by AuFON and Ag-coated insect wings. Finally, the AgNPs modified by PEG polymers were tested for intracellular application using HeLa cancer cells. Metallation of H2TMPyP served to probe the accessibility of PEG- AgNPs surface. The results proved that the accessibility...
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Loudness Index Measuring
Hloušek, Pavel ; Mach, Václav (referee) ; Kurc, David (advisor)
This thesis deals with noise measuring. There are described methods of measurement and evaluation of noise, focusing on Steven’s loudness calculation method. Basic concepts of acoustics are provided in the first part. There are also mentions measurement of noise using microphones and sound level meter. The second part is focused on the practical solution of thesis. In which it is made measurement of noise using sound level meter NTi Acoustilizer and implementation of the graphical user interface for calculation of the Steven’s loudness index in Matlab.
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Assessment of noise exposure at different ways of rearing water fowl.
BRAUN, Petr
This Bachelor´s work describes the evaluation of noise exposure at different ways of breeding water fowl. Breed selected for measuring the goose Czech. The aim is to measure and verify the intensity of noise arising from the farms and backyard and evaluate that noise acting on the surroundings. The basis is to implement the measurement noise level in backyard farms and are growing in a farm really out of the growing season for selected habitats and compare readings with legislative and hygiene standards. If permissible limits are exceeded measured values, it will be essential to propose the necessary restrictive measures.
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