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Application of (chir)optical analytical methods in the characterization of nonracemic helically chiral aromatics
Vilím, Vojtěch ; Hraníček, Jakub (advisor) ; Dian, Juraj (referee)
This master thesis is focused on preparation and characterization of thin layers for organic electronics forming a binary system, composed of achiral small conductive molecules (TIPS-pentacen) and chiral helicenes with various electronic density of their aromatic scaffold. Using organic electronics is innovative approach to manufacture electronic devices and compensate disadvantages of inorganic materials-based electronics. Induction of chirality into achiral organic electronics brings new possibilities for application in optical spintronics, optical quantum information processing and many others. Key words: thin layer, helicenes, circular dichroism, organic electronics
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Biological imaging by super-resolution microscopy
Adamová, Zuzana ; Komrsková, Kateřina (advisor) ; Benda, Aleš (referee)
zuzana_adamova_abstract_eng.txt[11.05.2017 20:26:29] Fluorescence microscopy is one of the most widely used imaging techniques in biological research. Despite its numerous advantages, it can be used only for studies of structures larger than 200 nanometres, due to diffraction limit caused by a wave nature of light. The value of 200 nanometres is the best reachable value of optical resolution, in other words, the smallest distance of two objects, which can be separately recognized by conventional optical systems. Up to the end of the 20th century it was therefore impossible to observe finer details of cells. However, recently several breakthrough imaging techniques, named super-resolution microscopy techniques, managed to bypass the diffraction limit and enabled biologists to study much more delicate structures, such as small organelles, virions, protein complexes or even particular proteins, while still using a visual light. This thesis introduces some selected super-resolution methods, explains briefly their principles and presents some of their applications in biology.
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