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Biologická a molekulární charakteristika Cryptosporidium suis-like
VLNATÁ, Gabriela
Cryptosporidium parvum VF383, more commonly known as Cryptosporidium suis-like, has been reported in humans, domesticated ruminants, and wild rats worldwide and described under several names including Cryptosporidium suis-like, based on its close phylogenetic relationship to C. suis. Unlike C. suis, however, it has never been detected in pigs. In the present work, Cryptosporidium suis-like originating from wild brown rats was not infectious for piglets or calves but was infectious for laboratory brown rats, BALB/c mice, and Mongolian gerbils. The prepatent period was 4-5 days for all rodents. The patent period was longer for rats (>30 days) than other rodents (<20 days). None of the rodents developed clinical signs of infection. In all rodents, life cycle stages were detected in the colon by histology and electron microscopy. Oocysts were morphometrically similar to those of C. parvum and smaller than those of C. suis, measuring 5.20 × 4.94 m. Phylogenetic analyses of SSU, actin, and HSP70 gene sequences revealed Cryptosporidium suis-like to be genetically distinct from, C. suis, and other described species of Cryptosporidium. Morphological, genetic, and biological data support the establishment of Cryptosporidium suis-like as a new species, and we propose the name Cryptosporidium occultus sp. n.
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