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Nucleotide variability in \kur{enol-1} gene of entomopathogenic nematodes \kur{Heterorhabditis bacteriophora} and \kur{Steinernema feltiae}
 HEJNÍČKOVÁ, Martina
The topic of this bachelor thesis is enol-1, parasitism-related gene in entomopathogenic nematodes. First part of this thesis deals with pattern of the enol-1 nucleotide variability. Nucleotide sequence of enol-1 was obtained from 9 strains of Heterorhabditis bacteriophora and 8 strains of Steinernema feltiae. I characterised and compared its structure and nucleotide variability. Phylogenetic analysis of enol-1 was done with closely related nematode coding sequences, acquired from internet databases. The second part of this thesis is devoted to virulence testing. Larvae of Galleria mellonella were infected with different strains of entomopathogenic nematodes, and their mortality was summarized.
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