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Charakteristika reprodukčních vlastností hřebců působících v inseminaci
BÚDOVÁ, Alena
One of the requirements for a successful insemination in horse breeding is the selection of quality stallions which produced ejaculate in enough quantities and of good quality. For this purpose were discovered methods of dealing with the evaluation of semen quality. In this dissertation was ejaculate evaluated by using an objective computerized method CASA, system SCA. Monitoring the quality of sperm was performed on 11 breeding stallions involved in artificial insemination fresh sperm in ZH Písek. 53 samples of ejaculate were evaluated. Volume, sperm concentration, total and progressive motility and sperm vitality were monitored. Volume of ejaculate was from 20 to 127.5 ml. Average values of sperm concentration for individual stallions were from 104.18 to 384.4 M/ml. Average values of total motility were moving by interval from 51.23 to 89.54 %. Average progressive motility was move from 12.99 to 47.12 %. At sperm vitality were discovered average results from 35.16 to 71.21 %. The average value of individual stallions was identical to the Decree of the Ministry of Agriculture, which sets minimum requirements for quality indicators for short-term preservation of semen. Also the quality of the semen of stallions was compared by age. The first group was made by stallions to 10 years old and the second group were older stallions. It was found that the younger stallions to 10 years had higher semen volume and greater sperm concentration. Stallions older over 10 years had higher levels of total and progressive motility and sperm vitality. Next ejaculate quality was evaluate by the time interval between samples. Regular taken from stallions is doing better ejaculate quality but the time interval between samples is important. The best values were found in samples with interval between the sampling 4 or more days. The worst quality of sperm was in the samples with the interval between 1-2 days.
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