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Vliv praziquantelu na raná vývojová stádia amura bílého
BÁRTOVÁ, Lucie
Praziquantel belongs to a group of medicines called anthelmintics, which are used to control parasitic infections. The aim of this study was to assess the effect of praziquantel on hatching, mortality, ontogenetic development, growth, oxidative stress and antioxidant enzyme activity in the early life stages of grass carp (Ctenopharyngodon idella). To assess the toxicity of praziquantel for the early life stages of grass carp, an embryo-larval fish toxicity test (OECD test no. 210) was used. The toxic effects of praziquantel were investigated when exposed to four concentrations (1 mg/l, 2 mg/l, 4 mg/l, 6 mg/l) for 29 days. A negative effect on curling was not observed in any group. A negative effect on mortality was observed in fish exposed to the concentrations of praziquantel 2 mg/l, 4 mg/l and 6 mg/l. Groups exposed to praziquantel concentrations of 4 mg/l and 6 mg/l died during the test period. A negative effect on ontogenetic development was observed in fish exposed to praziquantel concentrations of 4 mg/l and 6 mg/l. A negative effect of praziquantel on the activity of glutathione-S-transpherase was observed in the group exposed to a praziquantel concentration of 2 mg/l. A concentration of 1 mg/l praziquantel can be considered as a safe concentration for the early life stages of grass carp in the case of long-term exposure.
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The effect of metabolite terbuthylazine-2-hydroxy on early life stages of marbled crayfish
PEŘINOVÁ, Eliška
Terbuthylazine belongs to the group of pesticides on triazine-based for use in agriculture as herbicides. Terbuthylazine-2-hydroxy is the main metabolite of terbuthylazine. The aim of this study was evaluate the effect of terbuthylazine-2-hydroxy on mortality, growth, occurrence the deformations and the development of early life stages, and levels of oxidative stress, assess the activity of antioxidant enzymes of marbled crayfish (Procambarus fallax f. virginalis) and also augment data for assessing the impact of this metabolite on the environment. For assess of effect of this metabolite was used embryo larval toxicity test with crayfish. The effect of terbuthylazine-2-hydroxy was evaluated in four concentrations (0.75 g.l-1, 75 g.l-1, 375 g.l-1 and 750 g.l-1) for 62 days. Terbuthylazine-2-hydroxy in concentrations 375 g.l-1 and 750 g.l-1 caused decrease of activity superoxide dismutase (SOD) and the levels lipid peroxidation (measured as TBARS) and inhibition of growth. Inhibition of growth was also observed in the group exposed to 75 g.l-1 of terbuthylazine-2-hydroxy. Additionally, there was delay in ontogenetic development of crayfish exposed to concentration 750 g.l-1. The real tested concentration of 75 g.l-1 terbuthylazine-2-hydroxy had no effect to monitored indices in the early life stages of marbled crayfish. Terbuthylazine-2-hydroxy caused oxidative damage of cellular lipids and proteins, changes in antioxidant activity, reduction of growth, delay in ontogenetic development of marbled crayfish.
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