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National Repository of Grey Literature 1 records found  Search took 0.01 seconds. 
Detection of phytoplasmas using PCR amplification of selected fragments from the phytoplasma genome
 MARKOVÁ, Jaroslava
Phytoplasmas are bacterial pathogens of plants. They can cause devastating losses of yield of diverse crops. It is necessary to find reliable detection technique for detection of phytoplasmas disease. The easiest and the cheapest technique is just PCR (polymerase chain reaction) which is used to selective amplification of selected parts of the molecule DNA. The aim of this bachelor thesis is to find of optimal primers to amplification of selected fragments of phytoplasmas genome in one-step PCR and use this technique in detection of phytoplasmas. A collection of phytoplasmas from professor Bertaccini with 37 samples was used for this study. It was tested 16 natural samples by optimized primers. Phytoplasma was detected in strawberry root. Research was supported by the Czech Science Foundation, Grant No. GP 522/09/P545.
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