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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Improved demethylation in ecological epigenetic experiments: Testing a simple and harmless foliar demethylation application
 DVOŘÁKOVÁ, Hana
Spray application of 5azacytidine on established plant seedlings was tested for its demethylating efficiency. It represents a novel method for plant experimental demethylation with a potentially lower negative impact on plant development compared to the traditional application of the demethylating agent through germination of seeds in its solution. Further, the 5azacytidine spray application was used in practice to erase the epigenetic memory in offspring of Taraxacum brevicorniculatum plants from different competitive conditions. The impact of parental competition on the juvenile phenotype was estimated by measuring growth related traits, while the experimental demethylation allowed for evaluating the significance of DNA methylation marks in bioticaly induced transgenerational effects in T. brevirorniculatum.
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Arsenic speciation by hydride generation coupled with atomic fluorescence spectrometry
 Marschner, Karel ; Musil, Stanislav ; Rychlovský, P. ; Dědina, Jiří
Post-column hydride generation for arsenic speciation was used and the demethylation of Arsenic species during hydride generation was investigated.
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